2012 Fiscal Year Final Research Report
Non-invasive measurement of aqueous humor components by dynamic light scattering technique
| Project/Area Number |
22791699
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Nihon University |
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Principal Investigator |
OSHIDA Taiki 日本大学, 医学部, 助教 (00459885)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 外科系臨床医学 / 眼科学 / 眼医工学 / 動的光散乱法 / 前房内組成 |
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| Research Abstract |
We developed a new instrument for non-invasive measurement of aqueous humor components by dynamic light scattering technique. We investigated an optimal additive to determine endotoxin concentration in the viscoelastic substances with minimal dilution factor. In the light scattering method, a 40-fold dilution with PBS (0.01M, pH 7.4) enables a determination of the endotoxin concentration in viscoelastic substances. In this condition, adding HSA can provide better recovery rates.
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