2011 Fiscal Year Final Research Report
Three-dimensional quantification of tumor microenvironment in tongue squamous cell carcinoma
| Project/Area Number |
22792022
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 歯学 / 病理学 / 舌癌 / 舌癌 / 転移 / 癌微小環境 / 組織立体構築 / 3次元形態計測 |
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| Research Abstract |
The present study aimed at gaining 3D quantitative information about carcinoma invasion pattern, vascular network and accumulating fibroblasts/macrophages in the microenvironment of tongue squamous cell carcinoma(SCC). During the approved 2-year period, 3D reconstruction was achieved using 100 serial histological sections that were prepared from 30 cases of SCC. Phenotypes and architecture of carcinoma parenchyma and stroma were characterized by stepwise immunohistochemistry in combination with antibodies(cytokeratin, Ki-67, CD31, CD105, CD34, CD68,αSMA, S100A4). The resulting 3D constructs provided spatial resolution enough to grasp individual cells, aberrant divergence and tortuosity of tumor vessels, carcinoma vascular invasion and stromal cells. Our experience proved that 3D reconstruction offers an essential tool to validate the spatial association of cancer cells, vascular endothelial cells, myofibroblasts(CAF) and macrophages(TAM).
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