Direct imaging of flow-induced oscillatory calcium rise in living bone

2011 Fiscal Year Final Research Report

• Project/Area Number: 22792051
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Okayama University
• Principal Investigator: ISHIHARA Yoshihito 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70549881)
• Project Period (FY): 2010 – 2011
• Keywords: 歯科矯正学

Research Abstract

Bone cells form a complex three-dimensional network consisting of osteoblasts and osteocytes embedded in a mineralized extracellular matrix. Ca^<2+> acts as a ubiquitous secondary messenger in various physiological cellular processes and transduces numerous signals to the cell interior and between cells. However, the intracellular Ca^<2+> dynamics of bone cells have not been evaluated in living bone. In the present study, we developed a novel ex-vivo live Ca^<2+> imaging system that allows the dynamic intracellular Ca^<2+> concentration([Ca^<2+>] i) responses of intact chick calvaria explants to be observed without damaging the bone network. Our live imaging analysis revealed for the first time that both osteoblasts and osteocytes display repetitive and autonomic[Ca^<2+>] i oscillations ex vivo.

Research Products

• [Journal Article] In situ imaging of the autonomous intracellular Ca^<2+> oscillations of osteoblasts and osteocytes in bone (2012)
  • Author(s): Yoshihito Ishihara, Yasuyo Sugawara, Hiroshi Kamioka, Noriaki Kawanabe, Hiroshi Kurosaka, Keiji Naruse, Takashi Yamashiro
  • Journal Title: Bone Volume: 50(4) Pages: 852-852
  • Peer Reviewed
• [Journal Article] Roles of Heparan Sulfate Sulfation in Dentinogenesis (2012)
  • Author(s): Satoru Hayano, Hiroshi Kurosaka, Takeshi Yanagita, Ina Kalus, Fabian Milz, Yoshihito Ishihara, Md. Nurul Islam, Noriaki Kawanabe, Masahiro Saito, Hiroshi Kamioka, Taiji Adachi, Thomas Dierks, Takashi Yamashiro
  • Journal Title: Journal of Biological Chemistry Volume: (in press)
  • Peer Reviewed
• [Journal Article] Stage-specific embryonic antigen-4 identifies human dental pulp stem cells (2012)
  • Author(s): Noriaki Kawanabe, Satoko Murata, Hiroaki Fukushima, Yoshihito Ishihara, Takeshi Yanagita, Emmy Yanagita, Mitsuaki Ono, Hiroshi Kurosaka, Hiroshi Kamioka, Tomoo Itoh, Takuo Kuboki, Takashi Yamashiro
  • Journal Title: Experimental Cell Research Volume: 318(5) Pages: 453-63
  • Peer Reviewed
• [Journal Article] The three-dimensional morphometry and cell-cell communication in the osteocyte network in chick and mouse embryonic calvaria (2011)
  • Author(s): Yasuyo Sugawara, Ryoko Ando, Hiroshi Kamioka, Yoshihito Ishihara, Tadashi Honjo, Noriaki Kawanabe, Hiroshi Kurosaka, Teruko Takano-Yamamoto, Takashi Yamashiro
  • Journal Title: Calcified Tissue International Volume: 88(5) Pages: 416-426
  • Peer Reviewed
• [Presentation] 生体ライブイメージングを用いた骨組織中における自律性細胞内カルシウムオシレーションの検討 (2011)
  • Author(s): 石原嘉人
  • Organizer: 第70回日本矯正歯科学会大会 & 第4回国際会議
  • Place of Presentation: 名古屋
  • Year and Date: 20111118-20
• [Presentation] マウス大腿骨中の三次元骨細胞ネットワークは外部環境によって変化する (2011)
  • Author(s): 菅原康代, 上岡寛, 石原嘉人
  • Organizer: 第70回日本矯正歯科学会大会 & 第4回国際会議
  • Place of Presentation: 名古屋
  • Year and Date: 20111018-20
• [Presentation] マウス大腿骨中の三次元骨細胞ネットワークは外部環境によって変化する (2011)
  • Author(s): 菅原康代, 上岡寛, 石原嘉人
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜
  • Year and Date: 20110930-1001
• [Presentation] Ex-vivo imaging of the autonomous intracellular Ca^<2+> oscillations of osteoblasts and osteocytes in bone (2011)
  • Author(s): Ishihara Y
  • Organizer: The ASBMR 33th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 20110916-20
• [Presentation] Stage-specific embryonic antigen-4 identifies human dental pulp stem cells (2011)
  • Author(s): Kawanabe N., Murata S., Fukushima H., Ishihara Y
  • Organizer: The ASBMR 33th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 20110916-20
• [Presentation] 超高圧電子顕微鏡を用いた骨基質と骨細胞突起の3次元再構築 (2010)
  • Author(s): 今井裕一, 上岡寛, 石原嘉人
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20100927-29
• [Presentation] 骨組織内ライブイメージングを用いた機械的刺激に対する細胞内カルシウム応答の検討 (2010)
  • Author(s): 石原嘉人
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20100927-29
• [Presentation] ヒト歯根膜細胞におけるギャップ結合を介した細胞間コミュニケーションについて (2010)
  • Author(s): 吉川祐介, 石原嘉人
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20100927-29
• [Presentation] 骨組織内のEx vivo蛍光イメージングを用いた機械的刺激に対する細胞内カルシウム応答のリアルタイム解析 (2010)
  • Author(s): 石原嘉人
  • Organizer: 第52回歯科基礎医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20100920-22
• [Presentation] 骨組織内カルシウムイメージングを用いた機械的刺激に対する細胞応答のリアルタイム解析 (2010)
  • Author(s): 石原嘉人
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20100721-23
• [Presentation] SSEA-4 identifies human periodontal ligament stem cells (2010)
  • Author(s): Kawanabe N., Murata S., Murakami K., Ishihara Y
  • Organizer: 88th International Association for Dental Research & American Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100714-17
• [Presentation] Direct Imaging of Flow-induced Oscillatory Calcium Rise in Living Bone (2010)
  • Author(s): Ishihara Y
  • Organizer: 88th International Association for Dental Research & American Association for Dental Research(IADR Hatton award competition)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100714-17
• [Presentation] 超高圧電子顕微鏡による骨細胞の観察 (2010)
  • Author(s): 上岡寛, Sakhr A Murshid, 石原嘉人
  • Organizer: 第66回日本顕微鏡学会
  • Place of Presentation: 名古屋
  • Year and Date: 20100523-26