2011 Fiscal Year Final Research Report
Examination of intracellular localization and transport pathway of bone sialoprotein.
| Project/Area Number |
22792100
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Periodontal dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
KOTO Naoko 日本大学, 松戸歯学部, 兼任講師 (70434090)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 歯学 / 歯周病 / 石灰化 / 骨シアロタンパク質 |
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| Research Abstract |
Bone sialoprotein(BSP) is expected one of the important protein for calcification of bone. In order to clear the mechanisms of it through BSP, we investigated regulation of BSP transcription and transport pathway of BSP proteins in cell. Here, after stimulation with bone morphogenetic protein 2(BMP-2) of C2C12 cells which were myoblast, we showed that Smad1 bound to HOX domain in BSP promoter sequence and that Smad1 and Runx2 bound to FRE domain. And also we detected that C2C12 cells expressed BSP protein to induce calcification. Now, we are observing intracellular localization in the cells by electron microscopy.
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Research Products
(2 results)
