2011 Fiscal Year Final Research Report
Degradation of biofilm by fructanase chimeric enzyme
| Project/Area Number |
22890210
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Social dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
KAKUTA Erika 鶴見大学, 歯学部, 学部助手 (30585469)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 歯学 / 酵素 / 細菌 / 遺伝子 / バイオテクノロジー |
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| Research Abstract |
The purposes of this study were to construct chimeric enzyme of dextranase and fructanase, and to degrade the biofilm effectively. The dextranase gene and fructanase gene from S. mutans UA159 were cloned and connected in same plasmid vector. These enzymes were expressed in Escherichia coli. The function of enzyme was investigated by Somogyi-Nelson method using dextran and fructan as substrates. The enzyme degraded dextran and fructan. These results suggested that these enzymes are useful tool for the degradation of biofilm.
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