The regulatory mechanism of sympathetic nerves on eosinophil-associated micr o-inflammation in animals with IBS

2022 Fiscal Year Research-status Report

• Project/Area Number: 22K15656
• Research Institution: Hyogo Medical University
• Principal Investigator: 段 ショウキ 兵庫医科大学, 薬学部, 博士研究員 (20910607)
• Project Period (FY): 2022-04-01 – 2025-03-31
• Keywords: SNS / IBS / Micro-inflammation / Visceral pain

Outline of Annual Research Achievements

This research aims to investigate the relationship and potential mechanism between sympathetic nervous system (SNS) and micro-inflammation. In this year, I established the maternal separation (MS) model to mimic IBS. This model showed visceral hypersensitivity to colorectal distention, which is the main pathophysiology of IBS. I confirmed that there is micro-inflammation in colon of MS rats at adulthood by flowcytometry method and IHC. Besides, I researched the SNS activity. The ELISA result showed that NE concentration in serum increased in the MS rats compared with control. Neural activation markers are higher in MS model rats compared with control. Taken together, I confirmed that MS rats showed over activation of SNS. In addition, I applied the 6-OHDA to degenerate the SNS, the pharmacological data suggested that SNS played a role in immune cell alteration in colon. I also started to establish the genetic modulation approach to specifically modulation SNS activity.

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

In this year, I established the IBS model with micro-inflammation and over activation of SNS by MS stress. I pharmacologically confirmed that SNS activation modulate immune alteration in colon. By those data, I achieved the first step of the project.

Strategy for Future Research Activity

In the next year, I will focus on the genetic modulation of peripheral SNS by DBH-cre mice with AAV-PHP.S infection. Using this method, I will specifically activate or inhibit the peripheral SNS and check the immune alteration.

Causes of Carryover

In this year, I did not attend the conference, so there is some budget left.
Next year, I will mainly use the funds for purchasing animals and consumables such as reagents for physiological analysis of animal individuals and tissues, genetic reagents, which are necessary to accumulate experimental data. The funds will also be used for attending conferences (the 46th Annual Meeting of the Japan Neuroscience Society) and covering the publication fees for research articles.