Development of pollen-food allergy syndrome model mouse focusing on the sensitization route

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K16263
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53050:Dermatology-related
• Research Institution: Hiroshima University
• Principal Investigator: Ogino Ryohei 広島大学, 医系科学研究科(薬), 助教 (00816035)
• Project Period (FY): 2022-04-01 – 2024-03-31
• Keywords: 花粉-食物アレルギー症候群 / 花粉症 / 食物アレルギー / 感作経路 / シラカバ花粉 / リンゴアレルギー

Outline of Final Research Achievements

Pollen-food allergy syndrome (PFAS) is a type of food allergy which caused by immunoglobulin (Ig)E cross-reactivity between pollen allergens and proteins contained in fruit or vegetable. Although many causative cross-reactive allergens have been identified, the factors to develop PFAS have not revealed. This study aimed to develop PFAS model mouse and to analyze the effect of pollen-sensitization route for fruit allergy development in the model. First, birch pollen protein extract (BPE) sensitized mice were developed by intraperitoneal injection of BPE, and apple proteins (AP) were intraperitoneally administered one week later as a cross-reactive allergen. At challenge test, AP injection could not cause a decrease in rectal temperature as an allergic symptom but induce mast cell degranulation as an allergic reaction. Additionally, plasma IgE antibody from BPE sensitized mice reacted with 30 kDa AP, and this reaction was inhibited by pre-incubation the plasma with BPE.

Free Research Field

食物アレルギー

Academic Significance and Societal Importance of the Research Achievements

環境抗原を感作の原因とする交差反応による食物アレルギーはPFASの他にもネコアレルギー患者における豚肉アレルギーなどが存在し、これらは花粉症やネコアレルギー患者の一部で発症する。また、主要な交差反応性抗原であるシラカバBet v 1にはシラカバ花粉症患者のほとんどが感作されており、一部のみでPFAS発症に至る要因として、花粉中の交差反応性抗原への感作とは別の要素が存在することが予想される。本研究ではその要因の解明に向け、PFASモデルマウスの作製を試みた。現在実施中である感作経路毎のアレルギー症状の比較は、PFAS発症要因の解明と、抗原と発症要因の回避によるアレルギー予防策につながると考える。