2022 Fiscal Year Research-status Report
Mathematical model for quantitatively analysis the pathophysiological characteristics of mouse photoreceptor cell
| Project/Area Number |
22K20514
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
Muangkram Yuttamol 立命館大学, 立命館グローバル・イノベーション研究機構(BKC), 研究員 (90962473)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | ion channels / ion homeostasis / ionic current model / simulation model / mathematical model / photoreceptors / mouse retina |
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| Outline of Annual Research Achievements |
We have constructed a novel mathematical model of the mouse photoreceptor to quantitatively describe the electrophysiological characteristics of ionic current dynamics and the changes in intracellular ion concentrations under a variety of light intensities. The new model can be used to explain the pathophysiological changes in electrophysiological properties due to mutations in the ionic current-encoding genes of photoreceptors. The results have been reported in the annual conferences, including JSMBE, LE, Kinki PS, and PSJ. Currently, we have developed a model to estimate the energy consumption&production of photoreceptors, which are linked to several retinal diseases. A better understanding of energy demand&supply may provide an effective tool for analyzing new therapeutic approaches.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
We submitted our first-year studies in the mathematical model of the mouse photoreceptor to be published in an international journal. The contents included electrophysiological characteristics, alterations of ion channels, and estimations of required energy in darkness and in light. Now, we are estimating the energy production of photoreceptors and the contributions of anaerobic glycolysis, aerobic glycolysis, and fatty acid oxidation. The expected results could assist in a better understanding of many retinal diseases such as retinitis pigmentosa, age-related macular degeneration, and retinal detachment. We attempt to present these results at international conferences and publish them in peer-reviewed international journals.
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| Strategy for Future Research Activity |
We aim to fulfill our plan in the second part of the mathematical model, focusing on energy metabolism in mouse photoreceptors. After we have finished matching energy consumption and production, we will estimate oxygen demand and supply. Lastly, we will adjust the model to simulate the patterns of characteristic changes in the ERG waveform for experimental and clinical approaches. The complete model will quantitatively provide a valuable tool for investigating the pathophysiological changes in retinal diseases. The results will predict the disease's etiological mechanisms, which serve as a practical guideline in developing new drug research. The results will be reported at domestic and international conferences and in peer-reviewed scientific journals.
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| Causes of Carryover |
In the first year, we focused on how to construct a new mathematical model of a mouse photoreceptor. The model initially started with a small scale of computation. However, a larger scale of calculation is required. We rescheduled the purchase of a fast computer with a fast CPU, software, and data recording needed in the second year. Furthermore, we submitted our research results to domestic and international conferences and peer-reviewed journals at the end of the first year. The results will be announced in the second year, and the payments are required after we receive the acceptance.
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