2023 Fiscal Year Annual Research Report
Label-free intracellular dynamics investigation of carrier-free nanoparticle-based drug delivery systems
| Project/Area Number |
22K20524
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2022-08-31 – 2024-03-31
|
| Keywords | Drug delivery / Nanoparticles / Raman psectroscopy / SERS / Single-cell endoscopy / SN-38 |
|---|
| Outline of Annual Research Achievements |
We analyzed the metabolic process of SN-38 nanoprodrug in cancer cells using the ZIF8-AgNW endoscopic probe developed in FY2022. The interaction between the released drug molecule SN-38 and intracellular substances was investigated by single cell SERS endoscopy. It was found that the SN-38 molecule was released from the nanoparticles within 3 to 6 hours after the particles were taken up by the cells, and penetrated into the cytoplasm and nucleus. However, even though no Raman signal from the SN-38 molecule alone was detected after 24 hours, cell death occurred after 24 to 48 hours, suggesting that SN-38 interacts with DNA and topoisomerase after 24 hours. The results reported here show that the developed endoscopy technique may play an important role in studying drug mechanisms of action.
|
