2022 Fiscal Year Research-status Report
Functionalized capillary device for mass spectrometry-based subcellular lipid profiling
| Project/Area Number |
22K20560
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| Research Institution | Kyushu University |
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Principal Investigator |
LIU CHENCHEN 九州大学, 理学研究院, 助教 (50963211)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | single cell analysis / subcellular analysis / nano ESI / capillary needle / mass spectrometry / cell membrane lipid / lipid raft / lipid profiling |
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| Outline of Annual Research Achievements |
The objective of this project is to develop a capillary device that can perform subcellular lipid profiling. This device will allow for minute sampling, lipid purification and separation, and nano electrospray ionization for mass spectrometry (MS) analysis. Our preliminary tests have shown that a lab-made capillary device can be used for standard lipid MS detection. The device successfully achieved minute sampling and nano electrospray ionization, which have been integrated into a single capillary device. Building on this promising outcome, we plan to integrate the function for lipid purification and separation into the capillary device as well.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The project plan consists of the following steps: (1) Fabrication of a capillary needle capable of minute sampling. (2) Fabrication of a capillary device for nano electrospray ionization in mass spectrometry analysis. (3) Development of solid phase extraction material for lipid purification and integration of the material with the capillary device. (4) Development of an electrophoretic method for in-capillary lipid separation. We have successfully achieved two of these steps, and the study is progressing smoothly.
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| Strategy for Future Research Activity |
To enhance the efficiency of lipid sample preparation and analysis, we plan to optimize the current device construction to allow for controllable solvent exchange. Furthermore, we aim to fabricate solid phase extraction materials for lipid purification. Upon completion of this step, we will explore the use of electrophoretic separation methods for lipid analysis. Ultimately, our goal is to develop a capillary device for subcellular lipid profiling that can integrate multiple functions, such as minute sampling, lipid purification, and nano electrospray ionization, into a single device.
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