2023 Fiscal Year Annual Research Report
Functionalized capillary device for mass spectrometry-based subcellular lipid profiling
| Project/Area Number |
22K20560
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| Research Institution | Kyushu University |
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Principal Investigator |
LIU CHENCHEN 九州大学, 理学研究院, 助教 (50963211)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | capillary needle / mass spectrometry |
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| Outline of Annual Research Achievements |
The goal of this project is to create a capillary device capable of conducting subcellular lipid profiling. We have developed a refined capillary device model that enables the mass spectrometry detection of standard lipid samples in an analyte-to-answer fashion. In brief, the capillary's inner wall is coated with a hydrophobic material for lipid extraction, while the outer wall is coated with a conductive material for electrospray ionization. Additionally, the device includes capillary holders designed to interface with a mass spectrometry instrument. Although this project still requires further studies to optimize performance, our results have revealed new opportunities and challenges related to trace sample detection.
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