2023 Fiscal Year Annual Research Report
Functional elucidation of m6A mRNA modification in spatial map formation in hippocampal CA1 pyramidal cells
| Project/Area Number |
22K20702
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Phasuk Sarayut 国立研究開発法人理化学研究所, 生命機能科学研究センター, 特別研究員 (70961580)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | m6A / YTHDF3 / YTHDF1 / Place Cells / Calcium Imaging / Two Photon Microscopy / Spatial Memory / Virtual Reality |
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| Outline of Annual Research Achievements |
Using a conditional knockout (cKO) mouse model to delete YTHDF3 from excitatory neurons, we monitored neuronal activity with GCaMP6f delivered via Adeno-Associated Virus (AAV) and two-photon calcium imaging. cKO mice showed reduced running periods during habituation compared to wild-type (WT) mice, but their licking behavior patterns were similar. Although YTHDF3 depletion affected learning during acclimatization, it did not interfere with spatial memory formation, suggesting further research is needed to understand YTHDF3's role in spatial learning and memory.
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