2023 Fiscal Year Annual Research Report
Exploring the exosome-nanosome combinatorial platform as a novel, universal, and ideal nanoparticle-based drug delivery system
| Project/Area Number |
22K20705
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| Research Institution | Hokkaido University |
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Principal Investigator |
タン ロジャー・サルバシオン 北海道大学, 先端生命科学研究院, 助教 (10951759)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | Exosome / Nanosome / Drug Delivery System / Nanoparticles |
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| Outline of Annual Research Achievements |
In the extracellular vesicles population produced by cells, exosomes only constitute a small portion of the population. This has been one of the limitations of studying exosomes. One of the optimization protocols necessary and employed in this research was to find a way to induce the production of a higher number of exosomes in cancer cells. After employing different strategies, we were able to successfully induce cancer cells to produce a higher number of exosomes by starving them. Production and isolation of exosomes from starved cancer cells were then optimized to yield a stably high number of exosomes. Starved cancer cells produced a higher number of exosomes (1.56e+15 particles per mL with an average particle size of 139.5 nm) compared to the fed cancer cells (1.51e+10 particles per mL with an average particle size of 106.5 nm). This finding enabled us to optimize the exosome production and yielded considerably more exosomes needed for the study.
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