2023 Fiscal Year Research-status Report
Study on single molecule mechanobiology of microtubule
| Project/Area Number |
22KF0208
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| Allocation Type | Multi-year Fund |
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| Research Institution | Kyoto University |
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Principal Investigator |
角五 彰 京都大学, 理学研究科, 教授 (10374224)
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| Co-Investigator(Kenkyū-buntansha) |
NASRIN SYEDA 京都大学, 理学研究科, 外国人特別研究員
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| Project Period (FY) |
2023-03-08 – 2025-03-31
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| Keywords | kinesin / Dynein / Microtubule / Mechanical stress / Mechano-transducer |
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| Outline of Annual Research Achievements |
The current research hypothesizes that microtubules are mechanotransducers of mechanical stress. In this study, a comprehensive understanding of the issue will be made. Last year the modulation of cargo transport in response to mechanical stress on the microtubule is a universal phenomenon independent of the motor protein by using kinesin was verified. Kinesin-driven transport of fluorescent quantum dots was carried out on microtubules fixed on a stretchable substrate. The effect of the mechanical stress on the cargo transport was systematically evaluated by applying compressive strain or tensile strain using the microstretcher chamber developed previously. Parameter analysis e.g., run length, velocities, pause frequencies, etc., from the fluorescence microscopy observation was done.
This year, we also verified if the modulation of cargo transport in response to mechanical stress is recovered if the applied stress on the microtubule was withdrawn. For this research, I will first apply compressive stress on the microtubule using my micro-stretcher and release the stress. Then I observed dynein or kinesin-driven cargo transport and compare it with the case of undeformed microtubules to explore the restoration of the transport dynamics.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Because the research is progressing as planned, it was concluded that the project was on track.
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| Strategy for Future Research Activity |
Next year, we will summarize the following questions: (1) How does the mechanical stress-induced deformation of microtubules affect kinesin-driven cargo transport? (2) Is the cargo transport dynamics restored when applied stress on the microtubule is withdrawn? (3) Can the altered transport dynamics of dynein be theoretically explained by techniques like molecular dynamics simulation? We are also planning to propose a mechanism that explain the relationship between mechanical stress and the dynamics of kinesin.
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| Causes of Carryover |
解析、分析作業に多くの時間を費やしたため。
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