2023 Fiscal Year Annual Research Report
血液凝固因子が関わる新規機能としての感染防御システムの解明：メダカからヒトへ
| Project/Area Number |
22KJ1527
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| Allocation Type | Multi-year Fund |
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| Research Institution | Nagoya University |
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Principal Investigator |
MENG QI 名古屋大学, 創薬科学研究科, 特別研究員(DC1)
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| Project Period (FY) |
2023-03-08 – 2024-03-31
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| Keywords | Fibrinogen / Medaka / Immune response / Infection study |
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| Outline of Annual Research Achievements |
The purpose of my research is the investigation of the novel function of the fibrinogen in the immune system. I established the fibrinogen knockout medaka which shows anemia with smaller number of red blood cells (RBCs) and more immature RBCs. I tried to use two gram-negative bacteria, Flavobacterium columnare and Aeromonas hydrophila, to infect the medaka (Oryzias latipes) through immersing. However, the expression level of several immune-related factors were not increased after infection in RT-qPCR result. Then, I changed the induction methods to the intraperitoneal injection. In this experiment, the lipopolysaccharide (LPS) which is derived from the gram-negative bacteria cell wall, was injected into medaka intraperitoneally. After injection, the immune response was successfully induced with the elevated expression of several pro-inflammatory cytokines, including IFN-gamma, TNF-alpha, hepcidin, etc. There's also significant difference between wildtype (WT) and fibrinogen gamma knockout (KO) medaka in IL-1 beta and complement C1q subunit C expression upon infection.
In order to investigate the role of fibrinogen and its interacting protein in these immune pathway, the immunoprecipitation WB and mass spectrometry was carried out using WT and KO medaka upon infection. Through the immunoprecipitation using anti-medaka fibrinogen gamma antibody, several possible interacting protein was detected including histone. In order to investigate the function of fibrinogen in interaction with the histone in immune system, further investigation is necessary.
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