2022 Fiscal Year Annual Research Report
Development of Ligand-tethered Cu-responsive protein labeling chemical tool for visualizing Cu-release at synaptic cleft
| Project/Area Number |
22J15226
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| Allocation Type | Single-year Grants |
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| Research Institution | Kyoto University |
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Principal Investigator |
CHENG RONG 京都大学, 工学研究科, 特別研究員(DC2)
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| Project Period (FY) |
2022-04-22 – 2024-03-31
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| Keywords | biometal / copper ion / cuprous ion / protein labeling / conditional proteomics / chemical proteomics |
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| Outline of Annual Research Achievements |
This year, several cuprous-ion-responsive protein labeling reagents (CuRs) have been synthesized, and the chemical structure has been optimized. The cuprous ions-responsive reactions of the optimal CuR have also been studied and validated by HPLC analysis. Moreover, the capability of CuR to label and profile proteins in response to cuprous ions in multiple living cell systems has also been confirmed by western blotting, imaging, and proteomics analysis.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The optimal cuprous-ion-responsive protein labeling reagent (CuR) has been successfully developed. The labeling mechanism of CuR has been elucidated. CuR has also been successfully applied to multiple living cell systems. Therefore, the research has progressed on the whole more than it was initially planned.
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| Strategy for Future Research Activity |
In 2023, I will continue to collect data for the publication of the research results obtained. In particular, I will apply the developed optimal CuR to the ATP7A knock-out cells to map the proteins associated with impaired accumulation and export of copper ions by mass spectrometry to study the regulation of copper ions in living systems with genetic misregulation. After this, I will synthesize ligand-tethered cuprous ion-responsive protein labeling reagent (LT-CuR) targeting AMPA receptors to study copper release in the synapse.
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Research Products
(1 results)
