2023 Fiscal Year Annual Research Report
Development of Ligand-tethered Cu-responsive protein labeling chemical tool for visualizing Cu-release at synaptic cleft
| Project/Area Number |
22KJ1889
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| Allocation Type | Multi-year Fund |
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| Research Institution | Kyoto University |
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Principal Investigator |
CHENG RONG 京都大学, 工学研究科, 特別研究員(DC2)
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| Project Period (FY) |
2023-03-08 – 2024-03-31
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| Keywords | cuprous ion / copper ion / biometal / protein labeling / conditional proteomics / chemical proteomics / bioconjugation |
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| Outline of Annual Research Achievements |
This year, I applied optimal CuR to ATP7A knock out cells and identified labeled proteins by mass spectrometry to study the misregulation of copper ions in living systems. I have also collected data to publish the results of this research, which I have submitted to a journal for review. Optimal CuR structure will be used to develop cuprous ion probes with organelle or synapse cleft localization.
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