2013 Fiscal Year Final Research Report
Elucidation of mechanisms of energy dissipation in proteins by time- and space-resolved observation
| Project/Area Number |
23350007
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MIZUTANI Yasuhisa 大阪大学, 理学(系)研究科(研究院), 教授 (60270469)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | ラマン分光法 / 生物物理 / 時間分解分光法 |
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| Research Abstract |
Excess energy generated in an active site in proteins transferred to and dissipates in the protein moiety. Ultraviolet resonance Raman (UVRR) spectroscopy can selectively monitor Raman bands of aromatic amino acid residues, such as tryptophan, tyrosine and phenylalanine. Anti-Stokes Raman intensity reflects the population in vibrationally excited states. Therefore, anti-Stokes UVRR intensity can be a direct probe of vibrational energy of residues in a protein. In this study, we observed the vibrational energy migration in cytochrome b562 and myoglobin by using anti-Stokes UVRR spectroscopy and elucidated distance dependence of the vibrational energy migration.
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Research Products
(12 results)
