2013 Fiscal Year Final Research Report
Research on Full-protocol Disease Marker Screening System using Integrated Lab-on-a-CD for Immunoassay
| Project/Area Number |
23350035
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | University of Hyogo |
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Principal Investigator |
UTSUMI Yuichi 兵庫県立大学, 高度産業科学技術研究所, 教授 (80326298)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEO Masahiro 兵庫県立大学, 大学院・工学研究科, 准教授 (40236443)
YUSA Shinichi 兵庫県立大学, 大学院・工学研究科, 准教授 (00301432)
UKITA Yoshiaki 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 助教 (40578100)
KATAOKA Masatoshi) 産業技術総合研究所, 健康工学研究部門, グループ長 (20224438)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | チップ分析 / 生体分析 / 免疫反応 / バイオリアクター / ELISA / マイクロ化学システム / Lab-on-a-chip |
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| Research Abstract |
This paper reports a new type of lab-on-a-CD device with three-dimensional microchannel networks and vertical capillary bundle structure. The device consist of the multiple lab-on-a-CD chips with conventional planer microchannels and vertical microchannels which constructed on the disks made of poly-dimethylsiloxane (PDMS) and poly-methylmethacrilrate (PMMA). The PMMA layer with vertical channel were fabricated by deep X-ray lithography using synchrotron radiation. We designed and fabricated the chips for aim of taking enzyme-linked immunosorbent assay(ELISA). Three-dimensional liquid transportation through Lab-on-a-CD structure is suggested by computational fluid dynamics and it is successfully demonstrated chips. Detection of immunogroblin G(IgG) from mouse is successfully demonstrated in the three-dimensional devices and it is notable that the result suggests quite high-sensitive implying detection limit down to 1ng/mL by primitive result.
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