2013 Fiscal Year Final Research Report
Development of Photochemical RNA Editing induced by Photothermal Effect
| Project/Area Number |
23350079
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
FUJIMOTO KENZO 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 教授 (90293894)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKAHARA Toshifumi 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 教授 (60207339)
SAKAMOTO Takashi 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 助教 (80423078)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 光架橋 / RNA編集 |
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| Research Abstract |
To improve the deamination reaction of cytosine in the photocrosslinked DNA double strand, the effect of additives on the deamination reaction was evaluated. The results indicated that diamine derivatives can accelerate the reaction in a concentration dependent manner. diamine derivatives having more than four carbons distance between two amine groups clearly accelerate the photochemical C-U transition reaction. For spermidine, the acceleration effect was caused by the interaction between spermidine and DNA double strand, although the increase of pH scarcely contributed to the acceleration effect. As the mammalian cells contain millimolars of spermidine and spermine, the acceleration of photochemical C-U transition, that was demonstrated in this study, might occur in mammalian cells. This provides a new design of artificial oligonucleotide having higher photo-editing activity.
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