2013 Fiscal Year Final Research Report
Structural basis and functional evolution of RNA maturation machinary
| Project/Area Number |
23350081
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Ehime University |
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Principal Investigator |
HORI Hiroyuki 愛媛大学, 理工学研究科, 教授 (20256960)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Tsutomu 東京大学, 大学院・工学系研究科, 教授 (20292782)
HIRATA Akira 愛媛大学, 大学院・理工学研究科, 講師 (60527381)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 核酸 / 酵素 / タンパク質 / ＲＮＡ |
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| Research Abstract |
The purpose of this study is physical and chemical understanding of RNA maturation system. The study were performed for three years. We mainly researched the relatively simple organism of three domains of life (eukaryotes, archaea and eubacteria). We chracterized several tRNA modification enzymes and tRNA splicing endonulceases by X-ray crystal structural study and biochemical methods. Furthermore, we investigated the network between modified nucleosides in tRNA and tRNA modification enzymes using gene disruptant strains. Moreover, we found distinct tRNA modifications in a thermo-acidophilic archaeon, Thermoplasma acidophilum.
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Research Products
(69 results)
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[Journal Article] The 2'-O-methylation status of a single guanosine controls transfer RNA-mediated Toll-like receptor 7 activation or inhibition2012
Author(s)
S. Jöckel, G. Nees, R. Sommer, Y. Zhao, D. Cherkasov, H. Hori, G. Ehm, M. Schnare, M. Nain, A. Kaufmann, and S. Bauer
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Journal Title
J. Exp. Med
Volume: 209
Pages: 235-241
Peer Reviewed
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[Presentation] The synthesis mechanism of a tRNA modification in thermophilic bacteria2013
Author(s)
Takuya Kawamura, Chie Tomikawa, Takayuki Ohira, Yasushi Inoue, Nobukazu Nameki, Natsuhisa Oka, Hiroki Taniguchi, Kaori Ando, Satoshi Ohno, Akihiko Yamagishi, Tsutom Suzuki, Takashi Yokogawa, and Hiroyuki Hori
Organizer
Riboclub 2013 Annual Meeting
Place of Presentation
Canada
Year and Date
20130922-24
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[Presentation] Thermoplasma acidophilum 由来tRNALeuにおいてArchaeosine が2カ所に形成されるメカニズムの解析2013
Author(s)
河村卓哉, 冨川千恵, 大平高之 , 井上仁, 行木信一, 岡夏央, 谷口浩輝, 安藤香織, 大野敏, 山岸明彦, 鈴木勉, 横川隆志, 堀弘幸
Organizer
第15回日本RNA学会年会
Place of Presentation
松山
Year and Date
2013-07-24
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[Presentation] Multisite specific tRNA methyltransferase Trm1 from a hyper-thermophilic eubacterium, Aquifex aeolicus2011
Author(s)
T. Awai, A. Ochi, Ihsanawati, T. Sengoku, S. Kimura, C. Tomikawa, T. Yokogawa, A. Hirata, Y. Bessho, T. Suzuki, S. Yokoyama and H. Hori
Organizer
Thermophiles 2011
Place of Presentation
Big Sky, Montana, USA
Year and Date
2011-09-12
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