2014 Fiscal Year Final Research Report
Investigation of successive assembly mechanism of photosynthetic complexes
| Project/Area Number |
23370024
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Plant molecular biology/Plant physiology
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011-04-01 – 2015-03-31
|
| Keywords | 光合成 / 葉緑体 / チラコイド膜 / 光化学系 / アンテナ / ステート遷移 / 酸素発生系 / キノン |
|---|
| Outline of Final Research Achievements |
Photosynthetic reaction center and light-harvesting antenna form a complex consisting of a number of subunits and cofactors. In the present study, structure and successive assembly of these complexes has been analyzed using biochemical and molecular biological methods. The number and function of LHCII associated to PSI complex have been determined and the major naphthoquinone has been assigned as 5’-hydroxyphylloquinone. In addition, assembly mechanism of PSI complex has been analyzed using greening experiments and overexpressor of Ycf3 and Ycf4, which are required for PSI assembly. Site-directed mutagenesis that replaces amino acid residues involved in oxygen-evolution has been carried out to find that D1-N298 is an essential residue for the activity and protection against photodamage.
|
| Free Research Field |
光合成
|
