2013 Fiscal Year Final Research Report
Development of fish diversity studies by next-generation mitogenome sequencing: From Sanger method to massively parallel method
| Project/Area Number |
23370041
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | University of the Ryukyus (2013)
The University of Tokyo (2011-2012) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKESHIMA Hirohiko 東京大学, 農学生命科学研究科, 助教 (50573086)
MIYA Masaki 千葉県立中央博物館, 研究員 (30250137)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 進化 / 集団・種多様性 / 遺伝的多様性 |
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| Research Abstract |
Whole mitochondrial genome (mitogenome) sequences are extremely useful for resolving phylogenies of animals including fishes. This study aims to establish efficient procedures to obtain mitogenome data from many samples including degraded ones by using "next-generation" massively parallel sequencing technologies. For samples in good condition, long-PCR amplification/purification and parallel tagged sequencing successfully provided mitogenome data from many samples simultaneously, suggesting that this is a rather easier method for such samples. For degraded samples, many segments from mitogenome were successfully captured by using the capture-on-beads method with double indexing. Thus, we conclude that efficient procedures have been established to have large mitogenome data from many samples including degraded ones by using next-generation sequencing technologies.
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Research Products
(8 results)
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[Journal Article] Evolutionary origin of the Scombridae (tunas and mackerels) : members of a Paleogene adaptive radiation with 14 other pelagic fish families2013
Author(s)
Miya, M., Friedman, M., Satoh, T.P., Takeshima, H., Sado, T., Iwasaki, W., Yamanoue, Y., Nakatani, M., Mabuchi, K., Inoue, J.G., Poulsen, J.Y., Fukunaga, T., Sato, Y. and Nishida, M
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Journal Title
PLOS ONE
Volume: 8
Pages: e73535
DOI
Peer Reviewed
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[Journal Article] MitoFish and MitoAnnotator : a mitochondrial genome database of fish with an accurate and automatic annotation pipeline2013
Author(s)
Iwasaki, W., Fukunaga T., Isagozawa R., Yamada K., Maeda Y., Satoh T.P., Sado T., Mabuchi K., Takeshima H., Miya, M. and Nishida M
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Journal Title
Molecular Biology and Evolution
Volume: 30
Pages: 2531-2540
DOI
Peer Reviewed
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[Journal Article] Mitogenomic sequences and evidence from unique gene rearrangements corroborate evolutionary relationships of myctophiformes (Neoteleostei)2013
Author(s)
Poulsen, J.Y., Byrkjedal, I., Willassen, E., Rees, D., Takeshima, H., Satoh, T.P., Shinohara, G., Nishida, M. and Miya, M
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Journal Title
BMC Evolutionary Biology
Volume: 13
Pages: 111
DOI
Peer Reviewed
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