2013 Fiscal Year Final Research Report
Mechanisms of core promoter recognition by general transcription factor TFIID
| Project/Area Number |
23370077
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Yokohama City University |
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Principal Investigator |
KOKUBO Tetsuro 横浜市立大学, その他の研究科, 教授 (10271587)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 転写調節 / 転写因子 / 基本転写因子 / 転写開始 / 出芽酵母 / TFIID / TAF / TBP |
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| Research Abstract |
TFIID, the largest GTF composed of TBP and 14 TAFs, plays a critical role in transcription from TATA-less promoters. In metazoans, several core promoter elements (CE) other than the TATA element are thought to be recognition sites for TFIID. However, it is unclear whether functionally homologous elements also exist in TATA-less promoters in yeast.
Here we develop a novel high-throughput screening system to measure gene expression in yeast by using VTC1 as a reporter gene. This method allows us to isolate several active and novel CE by screening a randomized oligonucleotide pool for the CYC1 promoter. The TAF N-terminal domain (TAND) of the TAF1 protein plays a significant role in transcriptional activation. TAND comprises two subdomains, TAND1 and TAND2, which interact with the concave and convex surfaces of TBP, respectively. In collaboration with other groups, we determine the crystal structure of the TAND-TBP complex that provides a novel insight into transcriptional activation.
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Research Products
(19 results)
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[Journal Article] Identification and recombinant analysis of botrocetin-2, a snake venom cofactor for von Willebrand factor-induced platelet agglutination2012
Author(s)
Yamamoto-Suzuki Y, Sakurai Y, Fujimura Y, Matsumoto M, Hamako J, Kokubo T, Kitagawa H, Kawsar SM, Fujii Y, Ozeki Y, Matsushita F, Matsui T
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Journal Title
Biochemistry
Volume: 51(26)
Pages: 5329-38
Peer Reviewed
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[Book] Essay; Mechanisms of Transcriptional Activation and Repression (p1210-1217). Definitions; Activation Domain Shielding (p4-6), ATAC (p49-50), Chromatin Helicase DNA binding (CHD) (p402-404), INO80 (p1033-1035), ISWI (p1058-1060), Mammalian HDAC (p1173-1174), PIC Disassembly (p1715), Set3C (p1932-1933), TAND (p2121-2122), Tra1p (p2218-2219) : Encyclopedia ofSystems Biology, W. Dubitzky, O. Wolkenhauer, K. H. Cho, H. Yokota (Eds.)2013
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[Book] Essay; Transcription in Eukaryote (p2239-2245). Definitions; Cofactors (p436-437), Core Promoter Elements (p502-504), General Transcription Factors (p813-814), Mediator (p1217-1218), PIC Assembly Pathways (p1714-1715), RNA Polymerase I, II and III (p1862-1863), SAGA (p1890-1891), Type 3 Promoters (p2306-2307) : Encyclopedia of Systems Biology, W. Dubitzky, O. Wolkenhauer, K. H. Cho, H. Yokota (Eds.)2013
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[Book] Essay; Transcription Initiation in Eukaryote (p2245-2251). Definitions; Immobilized Template Assay (p989-990), Phosphodiester Bond Formation (p1702-1703), Stalk of RNAPII (p1982-1984), TAF Paralogs (p2120-2121), TFIIA-Like Factor (ALF) (p2163), TRF1 (p2299-2300), TRF2 (p2300-2301), TRF3 (p2301-2302) : Encyclopedia ofSystems Biology, W. Dubitzky, O. Wolkenhauer, K. H. Cho, H. Yokota (Eds.)2013
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