2013 Fiscal Year Final Research Report
Characterization of the centrosomal kinases that regulate chromosome instability in cancer cells.
| Project/Area Number |
23370086
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
NOJIMA HIROSHI 大阪大学, 微生物病研究所, 教授 (30156195)
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| Co-Investigator(Renkei-kenkyūsha) |
YABUTA Norikazu 大阪大学, 微生物病研究所, 准教授 (10343245)
OKUZAKI Daisuke 大阪大学, 微生物病研究所, 助教 (00346131)
NAITO Yoko 大阪大学, 微生物病研究所, 特任研究員 (10553026)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 染色体不安定性 / 中心体 / キナーゼ / M期チェックポイント / Lats / GAK |
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| Research Abstract |
Tumor malignancy promotes cell invasion and metastasis, which is one of the factors that hamper effective cancer therapy. Tumor malignancy means that cancer cells frequently cause missegregation of chromosomes on every cell division to generate two daughter cells with distinct chromosome quantity; this cancer specific feature is called "chromosomal instability". In this study, we show that the centrosomal kinases, Lats1/2 and GAK, form three novel signaling pathways and regulate the stability of chromosomes: they are Aurora-A-Lats1/2-Aurora-B (ALB) and Chk1-Lats2-14-3-3-P-body (CLP) pathways for Lats1/2 and GAK-PP2A-B'gamma-Chk2/Condensin II (GBC) pathway for GAK. To realize efficient analysis, we generated knockout mice lacking Lats1, Lats2, GAK, Cyclin G1 and/or Cyclin G2 genes, and Lats2-defective cancer cells using TALEN/Crispr_Cas systems.
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Research Products
(42 results)
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[Journal Article] Analysis of genes causing hypertension and stroke in spontaneously hypertensive rats : gene expression profiles in the brain2014
Author(s)
Yoshida M, Watanabe Y, Yamanishi K, Yamashita A, Yamamoto H, Okuzaki D, Shimada K, Nojima H, Yasunaga T, Okamura H, Matsunaga H, Yamanishi H
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Journal Title
Int. J. Mol. Med
Volume: 33
Pages: 887-896
DOI
Peer Reviewed
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[Journal Article] Genetic analysis of genes causing hypertension and stroke in spontaneously hypertensive rats2013
Author(s)
Yamamoto H, Okuzaki D, Yamanishi K, Xu Y, Watanabe Y, Yoshida M, Y amashita A, Goto N, Nishiguchi S, Shimada K, Nojima H, Yasunaga T, Okamura H, Matsunaga H, Yamanishi H
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Journal Title
Int. J. Mol. Med
Volume: 31
Pages: 1057-1065
DOI
Peer Reviewed
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[Journal Article] Yes-associated protein isoform 1 (Yap1) promotes cardiomyocyte survival and growth to protect against myocardial ischemic injury2013
Author(s)
Del Re DP, Y ang Y, Nakano N, Cho J, Zhai P, Yamamoto T, Zhang N, Yabuta N, Nojima H, Pan D, Sadoshima J
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Journal Title
J Biol Chem
Volume: 288
Pages: 3977-88
DOI
Peer Reviewed
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[Journal Article] N-terminal truncation of Lats1 causes abnormal cell growth control and chromosomal instability2013
Author(s)
Yabuta N, Mukai S, Okamoto A, Okuzaki D, Suzuki H, Torigata K, Yoshida K, Okada N, Miura D, Ito A, Ikawa M, Okabe M, Nojima H
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Journal Title
J Cell Sci
Volume: 126
Pages: 508-20
DOI
Peer Reviewed
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[Journal Article] Cancer susceptibility and embryonic lethality in Mob1a/1b double-mutant mice2012
Author(s)
Nishio M, Hamada K, Kawahara K, Sasaki M, Noguchi F, Chiba S, Mizuno K, Suzuki SO, Dong Y, Tokuda M, Morikawa T, Hikasa H, Eggenschwiler J, Yabuta N, Nojima H, Nakagawa K, Hata Y, Nishina H, Mimori K, Mori M, Sasaki T, Mak TW, Nakano T, Itami S, Suzuki A
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Journal Title
J Clin Invest
Volume: 122
Pages: 4505-18
DOI
Peer Reviewed
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[Journal Article] Microarray analysis of tonsils of IgA nephropathy patients2011
Author(s)
Iwatani H, Iio K, Nagasawa Y, Yamamoto R, Horii A, Okuzaki D, Inohara H, Nojima H, Imai E, Rakugi H, Isaka Y
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Journal Title
Adv . Otorhinolaryngol
Volume: 72
Pages: 75-78
DOI
Peer Reviewed
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