Elucidation of molecular mechanism of rice blast resistance

2013 Fiscal Year Final Research Report

• Project/Area Number: 23380024
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Hokkaido University
• Principal Investigator: SONE Teruo 北海道大学, (連合)農学研究科(研究院), 准教授 (00333633)
• Co-Investigator(Kenkyū-buntansha): TERAUCHI Ryohei 公益財団法人岩手生物工学研究センター, 生命科学研究部, 研究部長 (50236981)
• Co-Investigator(Renkei-kenkyūsha): OSE Toyoyuki 北海道大学, 大学院・薬学研究院, 准教授 (80380525)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: いもち病 / イネ / 抵抗性 / 非病原性遺伝子

Research Abstract

Rice blast is the most important disease or rice. Blast is usually controlled by agrochemicals and resistant cultivars, but knowledge about molecular mechanisms of rice resistance toward blast is limited. This study aimed to elucidate the molecular mechanism of rice blast resistance by analyzing interaction of rice blast resistance gene Pia and blast virulence gene AVR-Pia. Results of this study indicated that AVR-Pia gene is started to be expressed before the penetration into rice cells, and after the secretion, AVR-Pia protein forms homodimer, and detected by rice Pia gene product. This study will contribute to further understanding of rice-rice blast interaction, which is the first step of resistance induction.

Research Products

• [Journal Article] Heterologous production, purification, and immunodetection of Magnaporthe oryzae avirulence protein AVR-Pia (2014)
  • Author(s): Y. Satoh, S. Miki, T. Ose, A. Oikawa, K. Maenaka, R. Terauchi, K. Asano and T. Sone
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: (in press)
• [Journal Article] Homologous recombination causes the spontaneous deletion of AVR-Pia in Magnaporthe oryzae (2013)
  • Author(s): Teruo Sone, et al
  • Journal Title: FEMS Microbiology Letters Volume: 339 Pages: 102-109
• [Journal Article] イネいもち病菌エフェクターと宿主 R 遺伝子の微妙な関係 (2011)
  • Author(s): 曾根輝雄
  • Journal Title: 化学と生物 Volume: 49 Pages: 292-294
• [Presentation] Expression analysis of AVR-Pia, avirulence gene of Magnaporthe oryzae (2014)
  • Author(s): Sornkom, W., Takeuchi, S., Miki, S., Satoh, Y., and Sone, T
  • Organizer: 日本植物病理学会平成26年度大会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2014-06-02
• [Presentation] イネいもち病菌の非病原性タンパク質 AVR-Pia の多量体化と機能の関係 (2014)
  • Author(s): 藤原志帆, 樋口裕也, 佐藤佑樹, 尾瀬農之, Thomas Croj, Nam-Soo Jwa, 浅野行蔵, 曾根輝雄
  • Organizer: 日本植物病理学会平成26年度大会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2014-06-02
• [Presentation] イネいもち病菌の非病原性タンパク質 AVR-Pia の相互作用の解析 (2014)
  • Author(s): 樋口裕也, 佐藤佑樹, 尾瀬農之, 曾根輝雄
  • Organizer: 日本農芸化学会平成26年度大会
  • Place of Presentation: 明治大学生田キャンパス(川崎市)
  • Year and Date: 2014-03-29
• [Presentation] イネいもち病菌の病原性変異に関する分子遺伝学的研究 (2013)
  • Author(s): 曾根輝雄
  • Organizer: 日本農芸化学会北海道支部講演会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2013-11-30
  • Invited
• [Presentation] Toward understanding evolution and function of Magnaporthe oryzae effectors AVR-Pia and AVR-Pik (2013)
  • Author(s): Terauchi R., Saitoh, H., Kanzaki, H., Fujisaki, K., Takagi, H., Yoshida, K., and Kamoun, S
  • Organizer: International Rice Blast Conference
  • Place of Presentation: Ramada Plaza Jeju Hotel, Jejudo, South Korea
  • Year and Date: 2013-08-20
  • Invited
• [Presentation] Expression analysis of AVR-Pia, avirulence gene of Magnaporthe oryzae (2013)
  • Author(s): Sornkom, W., Takeuchi, S., Miki, S., Satoh, Y., and Sone, T
  • Organizer: International Rice Blast Conference
  • Place of Presentation: Ramada Plaza Jeju Hotel, Jejudo, South Korea
  • Year and Date: 2013-08-20
• [Presentation] Important amino acid region for multimerization of AVR-Pia protein of Magnaporthe oryzae (2013)
  • Author(s): Higuchi, T., Satoh, Y., Ose, T., and Sone, T
  • Organizer: International Rice Blast Conference
  • Place of Presentation: Ramada Plaza Jeju Hotel, Jejudo, South Korea
  • Year and Date: 2013-08-20
• [Presentation] Recombinant AVR-Pia Protein : Structure, biological activity, and application immunological detection of native AVR-Pia in Magnaporthe oryzae (2013)
  • Author(s): Satoh, Y., Ose, T., Kamiya, M., Terauchi, R., and Sone, T
  • Organizer: International Rice Blast Conference
  • Place of Presentation: Ramada Plaza Jeju Hotel, Jejudo, South Korea
  • Year and Date: 2013-08-20
• [Presentation] イネいもち病菌の非病原性タンパク質 AVR-Pia の局在解析 (2013)
  • Author(s): 竹内紗央里, 三木慎介, 佐藤佑樹, 曾根輝雄
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 岐阜大学(岐阜市)
  • Year and Date: 2013-03-29
• [Presentation] イネいもち病菌の非病原性タンパク質 AVR-Pia タンパク質の多量体化に関与する領域の探索 (2013)
  • Author(s): 樋口裕也, 佐藤佑樹, 尾瀬農之, 曾根輝雄
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 岐阜大学(岐阜市)
  • Year and Date: 2013-03-29
• [Presentation] 組換え AVR-Pia タンパクは Pia イネに HR 様反応を誘導する (2013)
  • Author(s): 佐藤佑樹, 尾瀬農之, 寺内良平, 曾根輝雄
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 岐阜大学(岐阜市)
  • Year and Date: 2013-03-29
• [Presentation] Homologous recombination causes the spontaneous deletion of AVR-Pia in Magnaporthe oryzae (2013)
  • Author(s): T. Sone, S. Takeuchi, S. Miki, Y. Sato, K. Ohtsuka, A. Abe, K. Asano
  • Organizer: 27th Fungal Genetics Conference
  • Place of Presentation: Asilomar Conference Grounds, CA (USA)
  • Year and Date: 2013-03-12
• [Presentation] Magnaporthe oryzae AVR-Pia protein : induction of resistance reaction in Pia rice by the recombinant AVR-Pia and preparation of anti-AVR-Pia antibody (2013)
  • Author(s): Y. Satoh, T. Ose, R. Terauchi, T. Sone
  • Organizer: 27th Fungal Genetics Conference
  • Place of Presentation: Asilomar Conference Grounds, CA (USA)
  • Year and Date: 2013-03-12
• [Presentation] 感染中のいもち病菌が分泌するAVR-Pia タンパク質はごく微量である (2012)
  • Author(s): 佐藤佑樹, 尾瀬農之, 寺内良平, 曾根輝雄
  • Organizer: 糸状菌分子生物学コンファレンス
  • Place of Presentation: ウインクあいち (名古屋)
  • Year and Date: 2012-11-12
• [Presentation] Strategy of the rice blast fungus to deal with blast resistant cultivars of rice (2012)
  • Author(s): Teruo Sone
  • Organizer: International conference on biosciences and biotechnology
  • Place of Presentation: Udayana University, Bali (Indonesia)
  • Year and Date: 2012-09-20
• [Presentation] Toward understanding Magnaporthe oryzae effector functions (2012)
  • Author(s): R., Terauchi
  • Organizer: 30th New Phytologist Symposium : Immunomodulation by plant-associated organisms
  • Place of Presentation: Stanford Sierra Conference Centre, Fallen Leaf Lake, CA(USA)
  • Year and Date: 2012-09-16
• [Presentation] Toward understanding Magnaporthe oryzae effector functions (2012)
  • Author(s): R., Terauchi
  • Organizer: 15th IS-MPMI CONGRESS
  • Place of Presentation: 京都国際会議場(京都市)
  • Year and Date: 2012-07-29
• [Presentation] Magnaporthe oryzae AVR-Pia protein : induction of resistance reaction in Pia rice and preparation of anti-AVR-Pia antibody (2012)
  • Author(s): Y. Sato, T. Ose, R. Terauchi, T. Sone
  • Organizer: 15th IS-MPMI CONGRESS
  • Place of Presentation: 京都国際会議場(京都市)
  • Year and Date: 2012-07-29
• [Presentation] Magnaporthe oryzae の非病原性タンパク質 AVR-Pia の多量体化に必要な領域の探索 (2012)
  • Author(s): 樋口裕也, 佐藤佑樹, 曾根輝雄
  • Organizer: 日本農芸化学会北海道支部夏期シンポジウム
  • Place of Presentation: 北海道大学水産学部講堂(函館市)
  • Year and Date: 2012-07-27
• [Presentation] 組換え AVR-Pia タンパクによる Piaイネの抵抗性誘導反応と抗 AVR-Pia 抗体の作成 (2012)
  • Author(s): 佐藤佑樹, 尾瀬農之, 寺内良平, 曾根輝雄
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-03-29
• [Presentation] AVR-Pia of Magnaporthe oryzae : expression, secretion and immunological detection (2012)
  • Author(s): Teruo Sone
  • Organizer: Japan-Australia Symposium on Plant Sciences for Agriculture 2011
  • Place of Presentation: 北海道大学農学部(札幌市)
  • Year and Date: 2012-01-19
• [Presentation] AVR-Pia of Magnaporthe oryzae : expression, secretion and mutation (2011)
  • Author(s): Teruo Sone
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-09-08
• [Presentation] AVR-Pia of Magnaporthe oryzae : expression, secretion and mutation (2011)
  • Author(s): Teruo Sone
  • Organizer: Asian Mycological Congress 2011
  • Place of Presentation: Incheon University, Incheon, Korea
  • Year and Date: 2011-08-10