2013 Fiscal Year Final Research Report

Identification and characterization of victorin-binding protein in oat plants

Research Project

Project/Area Number	23380026
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Plant pathology
Research Institution	Kagawa University
Principal Investigator	TADA Yasuomi 香川大学, 総合生命科学研究センター, 准教授 (40552740)
Co-Investigator(Kenkyū-buntansha)	AKIMITSU Kazuya 香川大学, 農学部, 教授 (80263888)
Project Period (FY)	2011-04-01 – 2014-03-31
Keywords	感染生理
Research Abstract	To identify victorin-binding proteins, a crude protein was extracted from the cell wall of the rice plant. The extract was fractionated by a gel filtration chromatography. Then, we have performed Biacore analysis to test if victorin binds to a cell wall protein. The Biacore positive fraction was mixed with the biotin-conjugated victorin (VicBio) and pulled down the victorin-binding proteins. A 15 kDa protein was specifically detected in VicBio-treated fraction, and mass spectrometry identified it as thioredoxin known as a reducing enzyme.