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2013 Fiscal Year Final Research Report

Development of a novel type worm-like DDS penetrable into tumor tissue

Research Project

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Project/Area Number 23390014
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionKyoto Pharmaceutical University

Principal Investigator

KOGURE Kentaro  京都薬科大学, 薬学部, 教授 (70262540)

Co-Investigator(Kenkyū-buntansha) TSUCHIY Hiroyuki  前京都薬科大学, 薬学部, 講師 (00403402)
HAMA Susumu  京都薬科大学, 薬学部, 講師 (60438041)
Project Period (FY) 2011-04-01 – 2014-03-31
KeywordsナノDDS / 癌細胞間隙 / 癌微小環境
Research Abstract

In this study, we attempted to develop a novel type worm-like DDS for overcoming drawbacks of conventional nanoDDS for anticancer therapy, such as, antigenicity of PEG, low cell affinity and low permeability into tumor tissue. As the results, we successfully developed charge-invertible liposomes, which can response to tumor microenvironment, and the capabilities of endosomal escape and cellular internalization and tumor accumulation comparable to PEG-liposomes were confirmed. Moreover, the worm-like nanostructures, which can penetrate into narrow space, were developed, and significantly high permeability into spheroids was achieved by improvement of physicochemical property of wick structure, modification with hyaluronidase and functional peptides. Then, integration of those functional liposomes, and evaluation of in vivo functionality was started.

  • Research Products

    (25 results)

All 2013 2012 2011 Other

All Journal Article (6 results) Presentation (16 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Development of a novel nanoparticle by dual modification with the pluripotential cell-penetrating peptide PepFect6 for cellular uptake, endosomal escape, and decondensation of an siRNA core complex2013

    • Author(s)
      Mitsueda A, Shimatani Y, Ito M, Ohgita T, Yamada A, Hama S, Gräslund A, Lindberg S, Langel U, Harashima H, Nakase I, Futaki S, Kogure K
    • Journal Title

      Biopolymers

      Volume: Vol.100 Pages: 698-704

    • DOI

      10.1002/bip.22310

  • [Journal Article] Protective effects of topical application of a poorly soluble antioxidant astaxanthin liposomal formulation on ultraviolet-induced skin damage2012

    • Author(s)
      Hama S, Takahashi K, Inai Y, Shiota K, Sakamoto R, Yamada A, Tsuchiya H, Kanamura K, Yamashita E, Kogure K
    • Journal Title

      J Pharm Sci

      Volume: Vol.101 Pages: 2909-2916

    • DOI

      10.1002/jps.23216

  • [Journal Article] Development of a novel drug delivery system consisting of an antitumor agent tocopheryl succinate2012

    • Author(s)
      Hama S, Utsumi S, Fukuda Y, Nakayama K, Okamura Y, Tsuchiya H, Fukuzawa K, Harashima H, Kogure K
    • Journal Title

      J Control Release

      Volume: Vol.161 Pages: 843-851

    • DOI

      10.1016/j.jconrel.2012.05.031

  • [Journal Article] Efficient intradermal delivery of superoxide dismutase using a combination of liposomes and iontophoresis for protection against UV-induced skin damage2012

    • Author(s)
      Kigasawa K, Miyashita M, Kajimoto K, Kanamura K, Harashima H, Kogure K
    • Journal Title

      Biol. Pharm. Bull

      Volume: Vol.35 Pages: 781-785

  • [Journal Article] A touch-and-go lipid wrapping technique in microfluidic channels for rapid fabrication of multifunctional envelope-type gene delivery nanodevices2011

    • Author(s)
      Kitazoe K, Wang J, Kaji N, Okamoto Y, Tokeshi M, Kogure K, Harashima H, Baba Y
    • Journal Title

      Lab. Chip

      Volume: Vol.11 Pages: 3256-3262

    • DOI

      10.1039/C1LC20392D

  • [Journal Article] invasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis2011

    • Author(s)
      Kajimoto K, Yamamoto M, Watanabe M, Kigasawa K, Kanamura K, Harashima H, Kogure K
    • Journal Title

      Int. J. Pharm

      Volume: Vol.403 Pages: 57-65

    • DOI

      10.1016/j.ijpharm.2010.10.021

  • [Presentation] Efficient cytoplasmic delivery of siRNA by tumoral pH sensitive nanoparticles via membrane fusion under tumor microenvironment2013

    • Author(s)
      Itakura S. Hama S. Nakai M. Nakayama K. Morimoto S. Tsuchiya H. Kogure K
    • Organizer
      40th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Hawaii, U.S.A
    • Year and Date
      20130700
  • [Presentation] A flexible nano carrier for siRNA delivery into tumor2013

    • Author(s)
      Kogure K. Shimatani Y. Uno K. Chon H.K. Hama S
    • Organizer
      40th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Hawaii, U.S.A
    • Year and Date
      20130700
  • [Presentation] 腫瘍低pH応答性ナノ粒子の効率的な細胞質送達メカニズムの解析2013

    • Author(s)
      濱 進, 板倉祥子, 中井麻友美, 中山佳代子, 森本智士, 小暮健太朗
    • Organizer
      第29回日本DDS 学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      20130700
  • [Presentation] 腫瘍集積性向上のための腫瘍微弱低pH応答性リポソームの改良と機能性評価2013

    • Author(s)
      森本智士, 濱 進, 板倉祥子, 中井麻友美, 中山佳代子, 土谷博之, 小暮健太朗
    • Organizer
      第29回日本DDS 学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      20130700
  • [Presentation] 腫瘍の微弱低pH に応答して細胞内取り込みが促進されるSAPS ペプチド修飾ナノ粒子の機能性評価2013

    • Author(s)
      中山佳代子, 濱 進, 板倉祥子, 中井麻友美, 森本智士, 大石利一, 土谷博之, 小暮健太朗
    • Organizer
      日本薬剤学会第28年会
    • Place of Presentation
      名古屋
    • Year and Date
      20130500
  • [Presentation] 腫瘍内部への浸透を目指した新規siRNA キャリアーの開発2013

    • Author(s)
      宇野晃平, 鄭 賢卿, 濱 進, 小暮健太朗
    • Organizer
      日本薬剤学会第28年会
    • Place of Presentation
      名古屋
    • Year and Date
      20130500
  • [Presentation] 柔軟な構造を有する組織浸透性siRNA キャリアーの開発2013

    • Author(s)
      小暮健太朗, 鄭 賢卿, 宇野晃平, 島谷悠里, 濵 進
    • Organizer
      第23回アンチセンスシンポジウム
    • Place of Presentation
      徳島
    • Year and Date
      20130100
  • [Presentation] Development of a novel drug delivery system for combination therapy in cancer2012

    • Author(s)
      Hama S. Utsumi S. Nakayama K. Okamura Y. Tsuchiya H. Fukuzawa K. Harashima H. Kogure K
    • Organizer
      第6回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      20121100
  • [Presentation] 腫瘍環境の微弱なpH に応答するペプチドを用いた薬物キャリアーの開発2012

    • Author(s)
      板倉祥子, 濱 進, 中井麻友美, 中山佳代子, 森本智士, 大石利一, 土谷博之, 小暮健太朗
    • Organizer
      第62回日本薬学会近畿支部総会・大会
    • Place of Presentation
      兵庫
    • Year and Date
      20121000
  • [Presentation] 抗癌剤トコフェロールコハク酸を用いた新規ドラッグデリバリーシステムの開発2012

    • Author(s)
      濱 進, 土谷博之, 小暮健太朗
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      20120900
  • [Presentation] Develpopment of nobel tumoral pH responsive nanoparticles for cancer therapy2012

    • Author(s)
      Hama S. Itakura S. Nakai M. Nakayama K, Morimoto S. Tsuchiya H. Kogure K
    • Organizer
      the 39th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Quebec city, Canada
    • Year and Date
      20120700
  • [Presentation] 組織狭小空間への送達を目指したsiRNA キャリアーの開発2012

    • Author(s)
      小暮健太朗, 島谷悠里, 宇野晃平, 土谷博之, 濱 進
    • Organizer
      第28回日本DDS 学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      20120700
  • [Presentation] 癌治療のための微弱pH 変化応答性薬物キャリアの開発2012

    • Author(s)
      森本智士, 濱 進, 板倉祥子, 中井麻友美, 中山佳代子, 土谷博之, 小暮健太朗
    • Organizer
      第28回日本DDS 学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      20120700
  • [Presentation] ナノ粒子の膜物性制御による腫瘍低pH下の癌細胞への効率的な薬物送達2011

    • Author(s)
      濱 進
    • Organizer
      第9回がんとハイポキシア研究会
    • Place of Presentation
      東京
    • Year and Date
      20111100
  • [Presentation] 腫瘍環境応答性ナノキャリアーの開発2011

    • Author(s)
      小暮健太朗, 板倉祥子, 中井麻由美, 中山佳代子, 森本智士, 濱 進
    • Organizer
      第33回生体膜と薬物の相互作用シンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      20111100
  • [Presentation] Development of a flexible siRNA carrier penetrable into tumor2011

    • Author(s)
      Kogure K, Shimatani Y, Uno K, Hama S
    • Organizer
      the IUMRS-ICA 2011, 12th International Conference in Asia
    • Place of Presentation
      台湾
    • Year and Date
      20110900
  • [Remarks]

    • URL

      http://labo.kyoto-phu.ac.jp/bukka/

  • [Patent(Industrial Property Rights)] pH応答性ペプチドを含むナノ粒子2012

    • Inventor(s)
      小暮健太朗, 濱 進
    • Industrial Property Rights Holder
      大鵬薬品工業株式会社
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      WO2012/147714A1
    • Filing Date
      2012-11-01
    • Overseas
  • [Patent(Industrial Property Rights)] pH応答性ペプチドを含むナノ粒子2011

    • Inventor(s)
      小暮健太朗, 濱 進
    • Industrial Property Rights Holder
      大鵬薬品工業株式会社
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      特願2011-097694
    • Filing Date
      2011-04-25

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Published: 2015-06-25  

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