2014 Fiscal Year Final Research Report
Regulatory mechanisms of MAPK signaling and its application to chemicalgenomics
Project/Area Number |
23390021
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Kinki University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KITA Ayako 近畿大学, 薬学部, 助教 (00388498)
MURAOKA Osamu 近畿大学, 薬学部, 教授 (20142599)
FUJIWARA Toshinobu 名古屋市立大学, 大学院薬学研究科, 教授 (80362804)
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Project Period (FY) |
2011-04-01 – 2015-03-31
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Keywords | MAPキナーゼ / 細胞内シグナル伝達 / ケミカルバイオロジー / 分子標的治療薬 / 分裂酵母 |
Outline of Final Research Achievements |
The purpose of this research project was to elucidate novel regulatory mechanisms of the Pmk1 MAPK signaling pathway in fission yeast and its application to genome-based drug discovery. We performed chemicalgenomics screens, utilizing the functional interaction between MAPK and calcineurin, and identified regulators (such as Cwg2, Rho4 and Rho5) and targets of Pmk1 MAPK in addition to several compounds that modify the ERK MAPK signaling pathway in mammals. Furthermore, we performed genome-wide identification of the genes involved in the sensitivities to FK506, Rapamycin and FTY720 and elucidated as-yet-unidentified functional interactions between MAPK and these gene products. Collectively, our data will contribute to the development of novel and effective therapeutic approaches for diseases associated with abrogated MAPK signaling activity.
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Free Research Field |
分子遺伝学、細胞生物学、ゲノム薬理学、分子細胞生物学、シグナル伝達、ゲノム創薬
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