Chemical Biology on the hybrid peptide-induce non-apoptotic cell death

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Drug development chemistry
• Research Institution: Sojo University
• Principal Investigator: KUNIYASU Akihiko 崇城大学, 薬学部, 教授 (90241348)
• Co-Investigator(Kenkyū-buntansha): MURATA Kazuyoshi 生理学研究所, 脳機能計測支援センター, 准教授 (20311201)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: ペプチドミメティックス / 細胞死 / 白血病

Research Abstract

We developed a hybrid peptide, which induces non-apoptotic cell death against leukemia cells. We determined that the mode of the peptide induced cell death is similar to necrosis.
In addition, we newly synthesized a series of the D-amino acid containing-peptide mimetic analogues of the hybrid peptide and evaluated the killing effect on the human leukemic cell lines. These peptides will be a useful tool for development of novel concept-based anti-leukemia agents.

Research Products

• [Journal Article] Crystallization and preliminary X-ray analysis of human MTH1 with a homogeneous N-terminus (2013)
  • Author(s): Koga Y., Inazato M., Nakamura T., Hashikawa, C., Chirifu M., Michi A., Yamashita T., Toma S., Kuniyasu A., Ikemizu S., Nakabeppu Y., Yamagata Y
  • Journal Title: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun Volume: 69 Pages: 45-48
  • Peer Reviewed
• [Journal Article] Intracerebral microinjection of interleukin-4/interleukin-13 reduces β-amyloid accumulation in the ipsilateral side and improves cognitive deficits in young amyloid precursor protein 23 mice (2012)
  • Author(s): Kawahara K., Suenobu M., Yoshida A., Koga K., Hyodo A., Ohtsuka H., Kuniyasu A., Tamamaki N., Sugimoto Y., Nakayama H
  • Journal Title: Neuroscience Volume: 207 Pages: 243-260
  • Peer Reviewed
• [Journal Article] Oxidized LDL and lysophosphatidylcholine stimulate plasminogen activator inhibitor-1 expression through reactive oxygen species generation and ERK1/2 activation in 3T3-L1 adipocytes (2011)
  • Author(s): Kuniyasu A., Tokunaga T., Yamamoto Y., Inoue S., Obama K., Kawahara K., Nakayama H
  • Journal Title: Biochim. Biophys. Acta Volume: 1811 Pages: 153-162
  • Peer Reviewed
• [Journal Article] Targeting neuropilin-1 in human leukemia and lymphoma (2011)
  • Author(s): Karjalainen K., Jaalouk D.E., Bueso- Ramos C.E., Zurita A.J., Kuniyasu A., Lichtiger B., O'Brien S., Kantarjian H.M., Cortes J.E., Koivunen E., Arap W., Pasqualini R
  • Journal Title: Blood Volume: 117 Pages: 920-927
  • Peer Reviewed
• [Presentation] ハイブリッドペプチドによる白血病細胞選択的ネクローシス様細胞死の誘導 (2014)
  • Author(s): 國安明彦, 坂口 隼, 藤川典子, 牧瀬正樹
  • Organizer: 日本薬学会第134回年会
  • Place of Presentation: 熊本市民総合体育館(熊本県熊本市)
  • Year and Date: 2014-03-28
• [Presentation] 核膜孔因子Nup50 とNup153 の相互作用とその核内輸送への役割 (2014)
  • Author(s): 牧瀬正樹, 福永晃祐, 猿渡康宏, 國安明彦
  • Organizer: 日本薬学会第134回年会
  • Place of Presentation: 熊本市民総合体育館(熊本県熊本市)
  • Year and Date: 2014-03-28
• [Presentation] Hybrid peptide tat-RAM13 induces a tumor-selective necrosis-like cell death in human leukemic cells (2014)
  • Author(s): Kuniyasu, A., Sakaguchi, H., Makise, M
  • Organizer: European Association of Cancer Research 2nd Special Conference "Cell Death in Cancer"
  • Place of Presentation: De Red Hoed (Amsterdam, オランダ国)
  • Year and Date: 2014-01-31
• [Presentation] 白血病細胞における抗悪性腫瘍ペプチドによるネクローシス様細胞死誘導 (2013)
  • Author(s): 國安明彦, 牧瀬正樹
  • Organizer: 日本癌学会第71回学術総会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2013-10-04
• [Presentation] 核膜孔因子Nup50とNup153 の相互作用とその核内輸送への役割 (2013)
  • Author(s): 牧瀬正樹, 國安明彦
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2013-09-11
• [Presentation] ミクログリア結合ペプチドの生細胞イメージングへの応用 (2012)
  • Author(s): 村木一徳, 牧瀬正樹, 國安明彦
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場(福岡県福岡市)
  • Year and Date: 2012-12-16
• [Presentation] 白血病細胞に特異的な非アポトーシス性細胞死の性状解析 (2012)
  • Author(s): 國安明彦, 中村優希, 藤川典子, 牧瀬正樹
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場(福岡県福岡市)
  • Year and Date: 2012-12-16
• [Presentation] カスパーゼに依存しない細胞死を誘導する白血病細胞選択的ペプチドミメティク化合物 (2012)
  • Author(s): 中村優希, 國安明彦
  • Organizer: 日本癌学会第71回学術総会
  • Place of Presentation: ロイトン札幌(北海道札幌市)
  • Year and Date: 2012-09-21
• [Presentation] 脂肪細胞における小粒子LDLによるMCP-1発現増大とその分子機序 (2012)
  • Author(s): 山田真梨子, 萱島知子, 國安明彦
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(北海道札幌市)
  • Year and Date: 2012-03-29
• [Presentation] A hybrid peptide-25mer induces a caspase-independent cell death in leukemic cell lines (2012)
  • Author(s): Kuniyasu, A., Nishimura, S
  • Organizer: European Association of Cancer Research Special Conference "Cell Death in Cancer"
  • Place of Presentation: De Rode Hoed (Amsterdam, オランダ国)
  • Year and Date: 2012-01-26
• [Presentation] ハイブリッドペプチドが誘導する白血病細胞に対する非アポトーシス細胞死 (2011)
  • Author(s): 國安明彦
  • Organizer: 日本癌学会第70回学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県名古屋市)
  • Year and Date: 2011-10-05