2013 Fiscal Year Final Research Report
Analysis of cohesin model in CYP3A4 gene and individual phenotypic status
| Project/Area Number |
23390035
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
IEIRI ICHIRO 九州大学, 薬学研究科(研究院), 教授 (60253473)
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| Co-Investigator(Kenkyū-buntansha) |
HIROTA Takeshi 九州大学, 大学院・薬学研究院, 助教 (80423573)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 遺伝子 / 発現制御 / 薬剤反応性 |
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| Research Abstract |
We showed that DNA methylation in CYP3A4 enhancer region is critically involved in determining the individual hepatic CYP3A4 mRNA level. Chromosome Conformation Capture (3C) assay suggested long-range cis-interaction between the enhancer region and the transcription start site. It is well known that miR-122 shows specific expression in human liver and asialoglycoprotein receptor (ASGP-R) is a liver-specific protein. We established the method using immuno-precipitation with polyclonal antibody against ASGP-R, to isolate the circulating cells showing miR-122 expression from peripheral blood. These results suggest that the isolated cells is from human liver cells. The established method can be used for the detection of human liver DNA methylation status using peripheral blood.
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