2014 Fiscal Year Final Research Report
Control of macropinocytosis by photomanipulation of Rac1 activity
| Project/Area Number |
23390039
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including histology/embryology)
|
|---|
| Research Institution | Kagawa University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAWAI Katsuhisa 香川大学, 医学部, 助教 (80534510)
EGAMI Youhei 香川大学, 医学部, 助教 (80432780)
MIYAKE Katsuya 香川大学, 医学部, 准教授 (30219745)
FUJII Makoto 香川大学, 医学部, 助教 (30398086)
|
|---|
| Project Period (FY) |
2011-04-01 – 2015-03-31
|
| Keywords | Rac1 / 分子スイッチ / マクロパイノサイトーシス / 蛍光イメージング / 光遺伝学 / マクロファージ |
|---|
| Outline of Final Research Achievements |
We attempted to characterize the activation and deactivation of Rac1 in macropinocytosis using microscopic photo-manipulation. Expression of genetically encoded photoactivatable(PA)-Rac1 in RAW264 macrophages enabled the local, reversible control of macropinocytosis using blue laser irradiation. Marked membrane ruffling and unclosed pre-macropinosomes were observed in the irradiated region of macrophages under the persistent activation of PA-Rac1. Although PI4,5P2 and actin accumulation were observed to this region, the recruitment of maturating endosome markers, such as PI3P and Rab21, was restricted until PA-Rac1 deactivation. After deactivating PA-Rac1 by ceasing irradiation, membrane ruffling immediately receded, and the macropinosomes acquired maturation markers. These data suggest that activation of Rac1 is sufficient to induce membrane ruffling and macropinocytic cup formation, but subsequent deactivation of Rac1 is required for macropinosome closure and further maturation.
|
| Free Research Field |
解剖学、細胞生物学
|
