Effects of insulin resistance-related factors on the stem cell functions in vivo.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390056
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: IMAMURA Takeshi 滋賀医科大学, 医学部, 准教授 (00552093)
• Co-Investigator(Kenkyū-buntansha): TORII Ryuzou 滋賀医科大学, 動物生命科学研究センター, 教授 (50106647)
• Co-Investigator(Renkei-kenkyūsha): YOSHIDA Yoshinori 京都大学, iPS 細胞研究所, 講師 (20447965) ; OKAMURA Tomio 滋賀医科大学, 医学部, 教授 (70152337) ; TAWA Masashi 滋賀医科大学, 医学部, 助教 (10510274)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 幹細胞 / インスリン抵抗性因子 / 糖尿病

Research Abstract

In this study, we planned to investigate whether the stem cell functions are damaged under the diabetic and/or insulin resistant conditions. Using the iPS cells established with the skin fibroblast of cynomolgus monkey, we identified the inhibitory effects of insulin resistant factor, IL-1beta on the ability of differentiation from iPS cells in vitro and in vivo. Thus, it is suggested that the stem cell functions are modified by the insulin resistant factors.

Research Products

• [Journal Article] Effects of atorvastatin, amlodipine, and their combination on vascular dysfunction in insulin-resistant rats (2014)
  • Author(s): Okamura T, Tawa M, Geddawy A, Shimosato T, Iwasaki H, Shintaku H, Yoshida Y, Masada M, Shinozaki K, Imamura T
  • Journal Title: J Pharmacol Sci Volume: 124 Pages: 76-85
  • URL: https://www.jstage.jst.go.jp/article/jphs/124/1/124_13178FP/_article
  • Peer Reviewed
• [Journal Article] The gut microbiota suppresses insulin-mediated fat accumulation via the short-chain fatty acid receptor GPR43 (2013)
  • Author(s): Kimura I, Ozawa K, Inoue D, Imamura T, Kimura K, Maeda T, Terasawa K, Kashihara D, Hirano K, Tani T, Takahashi T, Miyauchi S, Shioi G, Inoue H, Tsujimoto G
  • Journal Title: Nature Commun Volume: 4 Pages: 1829-1840
  • DOI: 10.1038/ncomms2852
  • Peer Reviewed
• [Journal Article] Influence of hypoxia on endothelium-derived NO-mediated relaxation in rat carotid, mesenteric and iliac arteries (2013)
  • Author(s): Tawa M, Shimosato T, Geddawy A, Imamura T, Okamura T
  • Journal Title: Pharmacology Volume: 91 Pages: 322-30
  • DOI: 10.1159/000351706
  • Peer Reviewed
• [Journal Article] Chronic Administration of Nicotine-Free Cigarette Smoke Extract Impaired Endothelium-Dependent Vascular Relaxation in Rats via Increased Vascular Oxidative Stress (2012)
  • Author(s): Shimosato T, Geddawy A, Tawa M, Imamura T, Okamura T
  • Journal Title: J Pharmacol Sci Volume: 118 Pages: 206-214
  • URL: https://www.jstage.jst.go.jp/article/jphs/118/2/118_11187FP/_article
  • Peer Reviewed
• [Journal Article] Contribution of nitric oxide in big endothelin-1-induced cardioprotective effects on ischemia/ reperfusion injury in rat hearts (2011)
  • Author(s): Tawa M, Fukumoto T, Ohkita M, Yamashita N, Geddawy A, Imamura T, Ayajiki K, Okamura T, Matsumura Y
  • Journal Title: J Cardiovasc Pharmacol Volume: 57 Pages: 575-578
  • DOI: 10.1097/FJC.0b013e31821325ad
  • Peer Reviewed
• [Journal Article] Impairment by hypoxia or hypoxia/reoxygenation of nitric oxide-mediated relaxation in isolated monkey coronary artery : the role of intracellular superoxide (2011)
  • Author(s): Tawa M, Yamamizu K, Geddawy A, Shimosato T, Imamura T, Ayajiki K, Okamura T
  • Journal Title: J Pharmacol Sci Volume: 116 Pages: 188-196
  • URL: https://www.jstage.jst.go.jp/article/jphs/116/2/116_11031FP/_article
  • Peer Reviewed
• [Presentation] Glycogen Synthase Kinase-3 beta differentially modifies early and late stages of adipogenesis from the pluripotent stem cells to mature adipocytes (2014)
  • Author(s): 今村武史
  • Organizer: 第87回日本薬理学会総会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2014-03-20
• [Presentation] Effects of diabetic conditions on the stem cell functions transplanted in diabetic monkeys (2014)
  • Author(s): 今村武史
  • Organizer: キーストン・シンポジウム
  • Place of Presentation: Keystone 米国 コロラド州
  • Year and Date: 2014-01-14
• [Presentation] インスリン抵抗性ラットにおける血管機能障害に及ぼすアトロバスタチンおよびアムロジピンの併用効果 (2013)
  • Author(s): 田和正志
  • Organizer: 第13回日本NO 学会学術集会
  • Place of Presentation: 沖縄県医師会館
  • Year and Date: 2013-06-28
• [Presentation] Effects of insulin resistance on adipogenic differentiation of the stem cells transplanted in cynomolgus monkeys (2013)
  • Author(s): 今村武史
  • Organizer: 第86回日本薬理学会総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2013-03-22
• [Presentation] Inhibition of Glycogen Synthase Kinase-3 beta enhances adipogenesis via the stimulation of glucose transport in mesenchymal stem cells (2013)
  • Author(s): 今村武史
  • Organizer: キーストン・シンポジウム
  • Place of Presentation: Keystone 米国 コロラド州
  • Year and Date: 2013-02-26
• [Presentation] Activity of fat cell differentiation in diabetic conditions (2012)
  • Author(s): 今村武史
  • Organizer: 第85回日本薬理学会総会
  • Place of Presentation: 国立京都国際会議場
  • Year and Date: 2012-03-16
• [Remarks]
  • URL: http://www.shiga-med.ac.jp/pharm/research.html