2013 Fiscal Year Final Research Report
Structure-based analysis of pathogenic activity of Helicobacter pylori CagA
| Project/Area Number |
23390076
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
HIGASHI Hideaki 北海道大学, 人獣共通感染症リサーチセンター, 教授 (20311227)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | タンパク質分子構造 / 細菌 / 胃がん |
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| Research Abstract |
Helicobacter pylori CagA protein is injected into gastric epithelial cells, where it interacts with cellular proteins such as SHP-2 and PAR1. CagA perturbs intracellular machineries involved in the regulation of cell growth and cell polarity through its C-terminal region containing Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs. To elucidate molecular basis for the pathogenic activity of CagA, we sought to determine three-dimensional structure of CagA. From results of proteolysis experiment, we found that CagA consists of the possible N-terminal and C-terminal structural domains. The CagA C-terminal fragment largely consists of an unfolded structure, although the fragment possesses an ability to bind with target molecules. Furthermore, at results of X-ray crystallographic analysis, the CagA N-terminal fragment indicate that the region has a structure comprised of three domains.
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Research Products
(13 results)
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[Journal Article] Genome Sequence of Bacillus anthracis outbreak strain in Zambia2014
Author(s)
Ohnishi N., Maruyama F., Ogawa H., Kachi H., Yamada S., Fujikura D., Nakagawa I., Hang'ombe B.M., Thomas Y., Mweene A.S., Higashi H.
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Journal Title
2011. Genome Announc
Volume: 2
Pages: e00116-14
DOI
Peer Reviewed
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[Journal Article] Caspase 3 Silencing Inhibits Biomechanical Overload-Induced Intervertebral Disk Degeneration2014
Author(s)
Yamada K., Sudo H., Iwasaki K., Sasaki N., Higashi H., Kameda Y., Ito M., Takahata M., Abumi K., Minami A., Iwasaki N.
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Journal Title
Am. J. Pathol.
Volume: 184
Pages: 753-764
DOI
Peer Reviewed
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[Journal Article] Tertiary structure-function analysis reveals the pathogenic signaling potentiation mechanism of Helicobacter pylori oncogenic effector CagA2012
Author(s)
Hayashi T., Senda M., Morohashi H., Higashi H., Horio M., Kashiba Y., Nagase L., Sasaya D., Shimizu T., Venugopalan N., Kumeta H., Noda N.N., Inagaki F., Senda T., Hatakeyama M.
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Journal Title
Cell Host Microbe
Volume: 12
Pages: 20-33
DOI
Peer Reviewed
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[Journal Article] Human - Animal Anthrax outbreak in the Luangwa valley of Zambia in 20112012
Author(s)
Hang'ombe B.M., Mwansa J.C.L., Muwowo S., Mulenga P., Kapina M., Musenga E., Squarre D., Mataa L., Thomas S.Y., Ogawa H., Sawa H., Higashi H.
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Journal Title
Trop. Doct.
Volume: 42
Pages: 136-139
DOI
Peer Reviewed
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