2013 Fiscal Year Final Research Report
Analysis of the roles of thioredoxin peroxidase family for adaptation to oxidative stresses in malaria parasites
| Project/Area Number |
23390098
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Satoru 獨協医科大学, 医学部, 准教授 (70275733)
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| Co-Investigator(Renkei-kenkyūsha) |
KANUKA Hirotaka 東京慈恵会医科大学, 医学部, 教授 (50342770)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 感染症 / マラリア / ストレス / レドックス / 遺伝子改変原虫 |
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| Research Abstract |
Phenotypes observed in the rodent malaria parasite Plasmodium berghei carrying a targeted knockout of thioredoxin peroxidase-family genes (2-Cys Prx KO and 1-Cys Prx KO) were investigated and have the following results: (1) Expression profiling of Prx mRNAs in 2-Cys Prx KO in the hepatic stage: qRT-PCR analysis revealed overexpression of other Prx mRNAs in the populations, indicating a mechanism underlying the response to oxidative stress in the hepatic-stage. (2) Defect in the hepatic-stage development of 2-Cys Prx KO: IFA analysis displayed smaller sized and fewer merozoite formation in the schizont than the parent strain (WT). (2) Defect in the blood-stage development of 1-Cys Prx KO: mice experiments showed lower parasite density of about 1-5% in the population than WT (>20%) during the first 7 days of infection.
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Research Products
(16 results)
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[Journal Article] Development of monoclonal antibodies that target 1-Cys peroxiredoxin and differentiate Plasmodium falciparum from P. vivax and P. knowlesi2013
Author(s)
Hakimi, H., Nguyen, TT., Suganuma, K., Masuda-Suganuma, H., Angeles, JM., Inoue, N., and Kawazu, S
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Journal Title
Trop. Med. Health
Volume: 41(2)
Pages: 55-59
DOI
Peer Reviewed
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[Journal Article] Expression profiles of peroxiredoxins in liver stage of the rodent malaria parasite Plasmodium berghei2013
Author(s)
Usui, M., Masuda-Suganuma, H., Fukumoto, S., Angeles, JM., Inoue, N., and Kawazu, S
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Journal Title
Parasitol. Int
Volume: 62(3)
Pages: 337-340
DOI
Peer Reviewed
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[Journal Article] Expression of cytosolic peroxiredoxins in Plasmodium berghei ookinetes is regulated by environmental factors in the mosquito bloodmeal2013
Author(s)
Turturice, BA., Lamm, MA., Tasch, JJ., Zalewski, A., Kooistra, RL., Schroeter, EH., Sharma, S., Kawazu, SI., and Kanzok SM
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Journal Title
PLoS Pathog
Volume: 9(1)
Pages: e1003136
DOI
Peer Reviewed
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[Journal Article] Plasmodium vivax and Plasmodium knowlesi: Cloning, expression and functional analysis of 1-Cys peroxiredoxin2012
Author(s)
Hakimi, H., Asada, M., Angeles, JM., Kawai, S., Inoue, N., and Kawazu, S
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Journal Title
Exp. Parasitol
Volume: 133(1)
Pages: 101-105
DOI
Peer Reviewed
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