Analysis of immune evasion system of Streptococcus pneumoniae

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390103
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: HAMADA Shigeyuki 大阪大学, 微生物病研究所, 特任教授 (60028777)
• Co-Investigator(Kenkyū-buntansha): KAWABATA Shigetada 大阪大学, 歯学研究科, 教授 (50273694) ; TERAO Yutaka 新潟大学, 医歯学系, 教授 (50397717)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 肺炎レンサ球菌 / 免疫回避 / 菌体表層タンパク / 貪食細胞

Research Abstract

Streptococcus pneumoniae is a common causative agent of pneumonia with high morbidity and mortality rates. In this study, we analyzed how Streptococcus pneumoniae evades host innate immunity. We revealed that pneumococcal a-enolase induce neutrophil extracellular trap formation and NETosis. Utilizing several biochemical assays and a recombinant procedure, a pneumococcal cell wall anchoring protein, PfbA, was found to function as an anti-phagocytic factor. In addition, we elucidated that S. pneumoniae invades human erythrocytes and escapes from neutrophil- and antibiotics-mediated killing.

Research Products

• [Journal Article] Development of a two-step multiplex PCR assay for typing of capsular polysaccharide synthesis gene clusters of Streptococcus suis (2014)
  • Author(s): Okura M, Lachance C, Osaki M, Sekizaki T, Maruyama F, Nozawa T, Nakagawa I, Hamada S, Rossignol C, Gottschalk M, Takamatsu D
  • Journal Title: J Clin Microbiol Volume: 52 Pages: 1714-1719
  • DOI: 10.1128/JCM.03411-13
  • Peer Reviewed
• [Journal Article] Crystal structure of PfbA, a surface adhesion of Streptococcus pneumoniae, provides hints into its interaction with fibronectin (2014)
  • Author(s): Beulin DS, Yamaguchi M, Kawabata S, and Ponnuraj K
  • Journal Title: Int J Biol Macromol Volume: 64 Pages: 168-173
  • DOI: 10.1016/j.ijbiomac.2013.11.035
  • Peer Reviewed
• [Journal Article] Streptococcus pneumoniae invades erythrocytes and utilizes them to evade human innate immunity (2013)
  • Author(s): Yamaguchi M, Terao Y, Mori-Yamaguchi Y, Domon H, Sakaue Y, Yagi T, Nishino K, Yamaguchi A, Nizet V, and Kawabata S
  • Journal Title: PLoS One Volume: 8 Pages: e77282
  • DOI: 10.1371/journal.pone.0077282
  • Peer Reviewed
• [Journal Article] Genetic analysis of capsular polysaccharide synthesis gene clusters from all serotypes of Streptococcus suis : potential mechanisms for generation of capsular variation (2013)
  • Author(s): Okura M, Takamatsu D, Maruyama F, Nozawa T, Nakagawa I, Osaki M, Sekizaki T, Gottschalk M, Kumagai Y, Hamada S
  • Journal Title: Appl Environ Microbiol Volume: 79 Pages: 2796-2806
  • DOI: 10.1128/AEM.03742-12
  • Peer Reviewed
• [Journal Article] Cysteine proteinase from Streptococcus pyogenes enables evasion of innate immunity via degradation of complement factors (2013)
  • Author(s): Honda-Ogawa M, Ogawa T, Terao Y, Sumitomo T, Nakata M, Ikebe K, Maeda Y, and Kawabata S
  • Journal Title: J Biol Chem Volume: 288 Pages: 15854-15864
  • DOI: 10.1074/jbc.M113.469106
  • Peer Reviewed
• [Journal Article] Group A streptococcal cysteine protease cleaves epithelial junctions and contributes to bacterial translocation (2013)
  • Author(s): Sumitomo T, Nakata M, Higashino M, Terao Y, and Kawabata S
  • Journal Title: J Biol Chem Volume: 288 Pages: 13317-13324
  • DOI: 10.1074/jbc.M113.459875
  • Peer Reviewed
• [Journal Article] MicroRNA fragments derived from Streptococcus pyogenes enable activation of neutrophil phagocytosis : in vitro study (2013)
  • Author(s): Ogawa T, Terao Y, Honda-Ogawa M, Hashimoto S, Ikebe K, Maeda Y, and Kawabata S
  • Journal Title: Microbes Infect Volume: 15 Pages: 212-218
  • DOI: 10.1016/j.micinf.2012.11.009
  • Peer Reviewed
• [Journal Article] Group A Streptococcus adheres to pharyngeal epithelial cells with salivary proline-rich proteins via GrpE chaperone protein (2012)
  • Author(s): Murakami J, Terao Y, Morisaki I, Hamada S, and Kawabata S
  • Journal Title: J Biol Chem Volume: 287 Pages: 22266-22275
  • DOI: 10.1074/jbc.M112.350082
  • Peer Reviewed
• [Journal Article] α-enolase of Streptococcus pneumonia induces formation of neutrophil extracellular traps (2012)
  • Author(s): Mori Y, Yamaguchi M, Terao Y, Hamada S, Ooshima T, and Kawabata S
  • Journal Title: J Biol Chem Volume: 287 Pages: 10472-10481
  • DOI: 10.1074/jbc.M111.280321
  • Peer Reviewed
• [Journal Article] Involvement of T6 pili in biofilm formation by serotype M6 Streptococcus pyogenes (2012)
  • Author(s): Kimura KR, Nakata M, Sumitomo T, Kreikemeyer B, Podbielski A, Terao Y, and Kawabata S
  • Journal Title: J Bacteriol Volume: 194 Pages: 804-812
  • DOI: 10.1128/JB.06283-11
  • Peer Reviewed
• [Journal Article] S-carboxymethylcysteine inhibits adherence of Streptococcus pneumoniae to human alveolar epithelial cells (2012)
  • Author(s): Sumitomo T, Nakata M, Yamaguchi M, Terao Y, and Kawabata S
  • Journal Title: J Med Microbiol Volume: 61 Pages: 101-108
  • DOI: 10.1099/jmm.0.033688-0
  • Peer Reviewed
• [Journal Article] Assembly mechanism of FCT region type 1 pili in serotype M6 Streptococcus pyogenes (2011)
  • Author(s): Nakata M, Kimura KR, Sumitomo T, Wada S, Sugauchi A, Oiki E, Higashino M, Kreikemeyer B, Podbielski A, Okahashi N, Hamada S, Isoda R, Terao Y, and Kawabata S
  • Journal Title: J Biol Chem Volume: 286 Pages: 37566-37577
  • DOI: 10.1074/jbc.M111.239780
  • Peer Reviewed
• [Journal Article] Biofilm formation or internalization into epithelial cells enable Streptococcus pyogenes to evade antibiotic eradication in patients with pharyngitis (2011)
  • Author(s): Ogawa T, Terao Y, Okuni H, Ninomiya K, Sakata H, Ikebe K, Maeda Y, and Kawabata S
  • Journal Title: Microb Pathog Volume: 51 Pages: 58-68
  • DOI: 10.1016/j.micpath.2011.03.009
  • Peer Reviewed
• [Journal Article] Epidemiological characterization of Streptococcus pyogenes isolated from patients with multiple onsets of pharyngitis (2011)
  • Author(s): Ogawa T, Terao Y, Sakata H, Ohkuni H, Ninomiya K, Ikebe K, Maeda Y, and Kawabata S
  • Journal Title: FEMS Microb Lett Volume: 318 Pages: 143-151
  • DOI: 10.1111/j.1574-6968.2011.02252.x
  • Peer Reviewed
• [Presentation] Streptococcus pyogenes が産生する FCT3 型線毛の発現調節機構の解析 (2014)
  • Author(s): 中田匡宣, 住友倫子, 浜田茂幸, 川端重忠
  • Organizer: ワークショップ「細菌の環境シグナル受容体と遺伝子調節ネットワーク」第87回日本細菌学会総会
  • Place of Presentation: 東京都江戸川区・タワーホール船堀
  • Year and Date: 20140326-28
• [Presentation] A 群レンサ球菌は宿主カルパインの活性化を介して上皮バリアを突破する( Group A Streptococcus translocates across an epithelial barrier via calpain activation ) (2013)
  • Author(s): 住友倫子, 中田匡宣, 寺尾豊, 川端重忠
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山市・岡山コンベンションセンター
  • Year and Date: 20130920-22
• [Presentation] A 群レンサ球菌の上皮バリア突破機構に関与する宿主プロテアーゼの解析 (2013)
  • Author(s): 住友倫子, 中田匡宣, 寺尾豊, 川端重忠
  • Organizer: 第22回Lancefield レンサ球菌研究会
  • Place of Presentation: 東京都港区・ホテル島根イン青山
  • Year and Date: 20130628-29
• [Presentation] Group A streptococcal pyrogenic exotoxin B cleaves epithelial junctions and contributes to bacterial translocation (2013)
  • Author(s): Sumitomo T, Nakata M, Higashino M, Terao Y, and Kawabata S
  • Organizer: 113th General Meeting of American Society for Microbiology
  • Place of Presentation: Colorado Convention Center , Denver, CO, USA
  • Year and Date: 20130518-21
• [Presentation] Cell wall anchoring mechanism of FCT region type 3 pili in Streptococcus pyogenes ( Streptococcus pyogenes が産生するFCT3 型線毛の細胞壁架橋機構の解析) (2013)
  • Author(s): 中田匡宣, 住友倫子, 寺尾豊, 浜田茂幸, 川端重忠
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市・幕張メッセ
  • Year and Date: 20130318-20
• [Presentation] Group A streptococcal cysteine protease cleaves epithelial junctions and contributes to bacterial translocation (2012)
  • Author(s): Sumitomo T, Nakata M, Higashino M, Terao Y, and Kawabata S
  • Organizer: Group A streptococcal cysteine protease cleaves epithelial junctions and contributes to bacterial translocation
  • Place of Presentation: Buyeo Lotte Resort, Buyeo, Korea
  • Year and Date: 20120913-14
• [Presentation] Assembly mechanism of T6 pili in serotype M6 Streptococcus pyogenes (2012)
  • Author(s): 中田匡宣, 住友倫子, 東野美晴, 岡橋暢夫, 浜田茂幸, 寺尾豊, 川端重忠
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市・長崎ブリックホール
  • Year and Date: 20120327-29
• [Presentation] Streptococcus pyogenes inhibits to form complement membrane attack complex (2012)
  • Author(s): 寺尾豊, 川端重忠
  • Organizer: ワークショップ「感染防御応答の多次元シグナルネットワーク」第85回日本細菌学会総会
  • Place of Presentation: 長崎市・長崎ブリックホール
  • Year and Date: 20120327-29
• [Presentation] A 群レンサ球菌の分泌型システインプロテアーゼである SpeB は上皮細胞間接着分子を分解する (2011)
  • Author(s): 住友倫子, 中田匡宣, 寺尾豊, 川端重忠
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜市・長良川国際会議場
  • Year and Date: 20110930-1002
• [Presentation] 赤血球存在下における肺炎レンサ球菌の免疫回避能 (2011)
  • Author(s): 大塚信太郎, 山口雅也, 寺尾豊, 川端重忠
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜市・長良川国際会議場
  • Year and Date: 20110930-1002
• [Presentation] Streptococcal pyrogenic exotoxin B contributes to cleavage of epithelial junctions (2011)
  • Author(s): Sumitomo T, Nakata M, Higashino M, Terao Y, and Kawabata S
  • Organizer: XVIII Lancefield International Symposium
  • Place of Presentation: Hotel Hilton Villa Igiea Palermo, Palermo, Italy
  • Year and Date: 20110904-08
• [Presentation] Streptococcus pneumoniae evades human innate immunity through invasion of erythrocytes (2011)
  • Author(s): Yamaguchi M, Terao Y, Yagi T, Nishino K, Yamaguchi A, and Kawabata S
  • Organizer: XVIII Lancefield International Symposium
  • Place of Presentation: Hotel Hilton Villa Igiea Palermo, Palermo, Italy
  • Year and Date: 20110904-08
• [Book] 感染・炎症・免疫, タイでコレラに挑む (2013)
  • Author(s): 岡田和久, 浜田茂幸
  • Total Pages: 43 (3): 42-54
  • Publisher: 羊土社
• [Book] 序 -注目される劇症型細菌感染症の現状と理解-.特集 1 「いわゆるヒト喰いバクテリアと劇症型感染症」 (2013)
  • Author(s): 川端重忠
  • Total Pages: 29(7): 26-27
  • Publisher: 医薬ジャーナル社 化学療法の領域
• [Book] 劇症型溶血性レンサ球菌感染症の発症に寄与する細菌因子.特集 1 「いわゆるヒト喰いバクテリアと劇症型感染症」 (2013)
  • Author(s): 住友倫子, 川端重忠
  • Total Pages: 29(7) : 48-55
  • Publisher: 化学療法の領域
• [Book] 病原性レンサ球菌の二成分制御系シグナル伝達機構.特集「細菌の病原遺伝子の発現調節機構」 (2013)
  • Author(s): 中田匡宣, 川端重忠
  • Total Pages: 29(1): 42-50
  • Publisher: 化学療法の領域
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/act/act_RCC-BacInfect.php