• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2013 Fiscal Year Final Research Report

Elucidation of the functions of miR-33a/b in cholesterol homeostasis

Research Project

  • PDF
Project/Area Number 23390211
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionKyoto University

Principal Investigator

ONO KOH  京都大学, 医学(系)研究科(研究院), 講師 (00359275)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Tomoyuki  関西医科大学, 医学部薬理学 (20362527)
Co-Investigator(Renkei-kenkyūsha) KAKEYA Hideaki  京都大学, 大学院・薬学研究科 (00270596)
SATO Fumiaki  京都大学, 大学院・薬学研究科 (20467426)
HORIE Takahiro  京都大学, 大学院・医学研究科循環器内科 (20565577)
Project Period (FY) 2011-11-18 – 2014-03-31
KeywordsマイクロRNA / HDL-C / 動脈硬化 / ABCA1 / SREBP-2 / SREBP-1 / 脂肪肝
Research Abstract

MicroRNAs (miRs) are small non-protein-coding RNAs that bind to specific mRNAs and inhibit translation or promote mRNA degradation. Recent reports, including ours, have indicated that miR-33 located within the intron of sterol regulatory element binding protein (SREBP) 2 controls cholesterol homeostasis and can be a potential therapeutic target for the treatment of atherosclerosis. Here we show that deletion of miR-33 results in marked worsening of high fat diet-induced obesity and liver steatosis. Using miR-33-/-Srebf1+/- mice we demonstrate that SREBP-1 is a target of miR-33 and that the mechanisms leading to obesity and liver steatosis in miR-33-/- mice involve enhanced expression of SREBP-1. These results elucidate a novel interaction between SREBP-1 and SREBP-2 mediated by miR-33 in vivo.

  • Research Products

    (28 results)

All 2014 2013 2012 2011 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (11 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] MicroRNAs and lipoprotein metabolism2014

    • Author(s)
      Horie T, Baba O, Kuwabara Y, Yokode M, Kita T, Kimura T, and Ono K
    • Journal Title

      J of Atherosclerosis and Thrombosis

      Volume: 21 Pages: 17-22

    • URL

      https://www.jstage.jst.go.jp/article/jat/21/1/21_20859/_article

    • Peer Reviewed
  • [Journal Article] Myocardial expression level of neural cell adhesion molecule correlates with reduced left ventricular function in human cardiomyopathy2014

    • Author(s)
      Nagao K, Sowa N, Inoue K, Tokunaga M, Fukuchi K, Uchiyama K, Ito H, Hayashi F, Makita T, Inada T, Tanaka M, Kimura T, and Ono K
    • Journal Title

      Circulation; Heart Failur

      Volume: 7 Pages: 351-8

    • DOI

      10.1161/CIRCHEARTFAILURE.113.000939

    • Peer Reviewed
  • [Journal Article] MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice2013

    • Author(s)
      Horie T, Nishino T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Sowa N, Yahagi N, Shimano H, Matsumura S, Inoue K, Marusawa H, Nakamura T, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T, and Ono K
    • Journal Title

      Nat Commun

      Volume: 4 Pages: 2883

    • DOI

      10.1038/ncomms3883

    • Peer Reviewed
  • [Journal Article] CREDO-Kyoto PCI/CABG registry cohort-2 investigators. Impact of polyvascular disease on clinical outcomes in patients undergoing coronary revascularization : An observation from the CREDO-Kyoto Registry Cohort-22013

    • Author(s)
      Morikami Y, Natsuaki M, Morimoto T, Ono K, Nakagawa Y, Furukawa Y, Sakata R, Aota M, Okada Y, Onoe M, Kawasuji M, Koshiji T, Nakajima H, Nishizawa J, Yamanaka K, Yamamoto H, Kimura T
    • Journal Title

      Atherosclerosis

      Volume: 228 Pages: 426-31

    • DOI

      10.1016/j.atherosclerosis.2013.04.005

    • Peer Reviewed
  • [Journal Article] Metastasis-associated protein, S100A4 mediates cardiac fibrosis potentially through the modulation of p53 in cardiac fibroblasts2013

    • Author(s)
      Tamaki Y, Iwanaga Y, Niizuma S, Kawashima T, Kato T, Inuzuka Y, Horie T, Morooka H, Takase T, Akahashi Y, Kobuke K, Ono K, Shioi T, Sheikh SP, Ambartsumian N, Lukanidin E, Koshimizu TA, Miyazaki S, Kimura T
    • Journal Title

      J Mol Cell Cardiol

      Volume: 57 Pages: 72-81

    • DOI

      10.1016/j.yjmcc.2013.01.007

    • Peer Reviewed
  • [Journal Article] MicroRNA-33-deficiency reduces the progression of atherosclerotic plaque in apoE-/- mice2012

    • Author(s)
      Horie T, Baba O, Kuwabara Y, Chujo Y, Watanabe S, Kinoshita M, Horiguchi M, Nakamura T, Chonabayashi K, Hishizawa M, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T, Ono K
    • Journal Title

      JAm Heart Assoc

      Volume: 1 Pages: e003376

    • DOI

      10.1161/JAHA.112.003376

    • Peer Reviewed
  • [Journal Article] Current concept of reverse cholesterol transport and novel strategy for atheroprotection2012

    • Author(s)
      Ono K
    • Journal Title

      J Cardiol

      Volume: 60 Pages: 339-43

    • DOI

      10.1016/j.jjcc.2012.07.014

    • Peer Reviewed
  • [Journal Article] MicroRNA 26b Encoded by the Intron of Small CTD Phosphatase (SCP) 1 Has an Antagonistic Effect on its Host Gene2012

    • Author(s)
      Sowa N, Horie T, Kuwabara Y, Baba O, Watanabe S, Nishi H, Kinoshita M, Takanabe-Mori R, Wada H, Shimatsu A, Hasegawa K, Kimura T, and Ono K
    • Journal Title

      Journal ofCellular Biochem.

      Volume: 113 Pages: 3455-65

    • DOI

      10.1002/jcb.24222

    • Peer Reviewed
  • [Journal Article] Increased microRNA-1 and microRNA-133a Levels in Serum of Patients with Cardiovascular Disease Indicate the Existence of Myocardial Damage2011

    • Author(s)
      Kuwabara Y, Ono K, Horie T, Nishi H, Nagao K, Kinoshita M, Watanabe S, Baba O, Kojima Y, Shizuta S, Imai M, Tamura T, Kita T, Kimura T
    • Journal Title

      Circulation : Cardiovascular Genetics

      Volume: 4 Pages: 446-454

    • DOI

      10.1161/CIRCGENETICS.110.958975

    • Peer Reviewed
  • [Journal Article] MicroRNA links obesity and impaired glucose metabolism2011

    • Author(s)
      Ono K
    • Journal Title

      Cell Res

      Volume: 21 Pages: 864-6

    • DOI

      10.1038/cr.2011.78

    • Peer Reviewed
  • [Journal Article] MicroRNAs and Cardiovascular Diseases2011

    • Author(s)
      Ono K, Kuwabara Y, Han J
    • Journal Title

      FEBS J

      Volume: 278 Pages: 1619-33

    • DOI

      10.1111/j.1742-4658.2011.08090.x

    • Peer Reviewed
  • [Journal Article] Comparison of Three-Year Clinical Outcomes after Sirolimus-eluting Stent Implantation among Insulin-treated Diabetic, Non-insulin-treated Diabetic and Non-diabetic Patients From the j-Cypher Registry2011

    • Author(s)
      Tada T, Kimura T, Morimoto T, Ono K, Furukawa Y, Nakagawa Y, Nakashima H, Ito A, Shiode N, Namura M, Inoue N, Nishikawa H, Nakao K, Mitsudo K
    • Journal Title

      Am J Cardiol

      Volume: 107 Pages: 1155-62

    • DOI

      10.1016/j.amjcard.2010.12.013

    • Peer Reviewed
  • [Journal Article] MicroRNA-27a regulates beta cardiac myosin heavy chain gene expression by targeting thyroid hormone receptor beta 1 in neonatal rat ventricular myocytes2011

    • Author(s)
      Nishi H, Ono K, Horie T, Nagao K, Kinoshita M, Kuwabara Y, Watanabe S, Takaya T, Tamaki Y, Takanabe-Mori R, Wada H, Hasegawa K, Iwanaga Y, Kawamura T, Kita T, Kimura T
    • Journal Title

      Mol Cell Biol

      Volume: 31 Pages: 744-55

    • DOI

      10.1128/MCB.00581-10

    • Peer Reviewed
  • [Presentation] MicroRNA-33 encoded by an intron of sterol regulatory element-binding protein-2 (Srebp2) regulates Srebp1 in vivo2014

    • Author(s)
      Horie T, Nishino T, Baba O, Kuwabara Y, Nakao T, Nishiga M, Usami S, Izuhara M, Sowa N, Yahagi N, Shimano H, Hasegawa K, Kume N Yokode M, Kita T, Kimura T, Ono K
    • Organizer
      The 18th International Vascular Biology Meeting
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-04-14
  • [Presentation] Cholesterol and Fatty acid regulation by miR-33a/b2014

    • Author(s)
      Ono K
    • Organizer
      Molecular Clock ~New waves of RNA regulation~
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-03-29
  • [Presentation] LncRNAs regulated by pressure-overload induce hypertrophy in neonatal mouse ventricular cardiomyocytes2013

    • Author(s)
      Kuwabara Y
    • Organizer
      American Heart Association Annual Scientific Sessions
    • Place of Presentation
      Dallas
    • Year and Date
      20131116-22
  • [Presentation] Cardiac-specific inhibition of kinase activity in calcium/calmodulin-dependent kinase kinase-・ leads to accerelated left ventricular remodeling and heart failure after transverse aortic constriction in mice2013

    • Author(s)
      Ono K
    • Organizer
      Cold spring harbor laboratory meeting
    • Place of Presentation
      NY, U.S.A
    • Year and Date
      20130813-17
  • [Presentation] MicroRNA-33 Deficiency Reduced Atherosclerosis Formation In Vivo2012

    • Author(s)
      Horie T, Baba O, Ono K, Kuwabara Y, Hasegawa K, Kume N, Yokode M, Kita T, and Kimura T
    • Organizer
      American Heart Association Annual Scientific Sessions 2012
    • Place of Presentation
      Los Angeles Convention Center
    • Year and Date
      20121103-07
  • [Presentation] MicroRNA-33-deficiency reduces the progression of atherosclerotic plaque in apoE-/- mice Cold spring harbor laboratory meeting2012

    • Author(s)
      Ono K
    • Organizer
      Regulatory and non-coding RNA
    • Place of Presentation
      NY, U.S.A
    • Year and Date
      20120828-0901
  • [Presentation] MicroRNA 33 Deficiency Reduces Atherosclerotic Plaque progression in ApoE Knockout Mice2012

    • Author(s)
      Baba O, Horie T, Nakamura T, Hasegawa K, Kume N, Yokode M, Kita T, Kimura T, and Ono K
    • Organizer
      European Society of Cardiology Congress 2012
    • Place of Presentation
      Munich - Germany
    • Year and Date
      20120825-29
  • [Presentation] Symposium 6 (Insulin resistance) MicroRNA regulation of lipid homeostasis2012

    • Author(s)
      Ono K
    • Organizer
      25th Spring Congress of Korean Diabetes Association
    • Place of Presentation
      Hotel Inter-Burgo Exco, Daegu
    • Year and Date
      2012-05-11
  • [Presentation] MicroRNA-1 and microRNA-133a elevations in serum of patients with cardiovascular disease indicate the existence of myocardial damage2011

    • Author(s)
      Kuwabara Y, Ono K, Horie T, Nishi H, Watanabe S, Kinoshita M, Baba O, Chujo Y, Nagao K, Kojima Y, Kita T, Kimura T
    • Organizer
      American Heart Association Annual Scientific Sessions
    • Place of Presentation
      Orlando, Florida
    • Year and Date
      20111112-16
  • [Presentation] Neural cell adhesion molecule is a cardioprotective factor upregulated by metabolic stress2011

    • Author(s)
      Nagao K, Ono K, Horie T, Tamaki Y, Iwanaga Y, Makita T, Inada T, Tanaka M, Kita T, Kimura T
    • Organizer
      European Society of Cardiology Congress
    • Place of Presentation
      Paris, France
    • Year and Date
      20110827-31
  • [Presentation] MicroRNA-33 Encoded by an Intron of Srebp2 Regulates HDL in vivo. 2011AVDRC International Symposium2011

    • Author(s)
      Ono K
    • Organizer
      Intervention of Vascular Aging : New Frontiers
    • Place of Presentation
      College of Medicine, Yeungnam University, Daegu, Korea
    • Year and Date
      2011-09-22
  • [Book] Annual Review循環器2014, 代謝と循環器疾患に関わるmicroRNA2014

    • Author(s)
      尾野亘
    • Total Pages
      35-41
    • Publisher
      中外医学社
  • [Book] Annual Review 糖尿病・代謝・内分泌2012, microRNAによるHDL代謝調節2012

    • Author(s)
      尾野亘
    • Total Pages
      80-85
    • Publisher
      中外医学社
  • [Remarks]

    • URL

      http://kyoto-u-cardio.jp/kisokenkyu/metabolic/

  • [Patent(Industrial Property Rights)] マイクロRNA溶出型ステント及びそれを用いた管腔治療2014

    • Inventor(s)
      尾野亘, 斉藤成達, 出原正康, 木村剛
    • Industrial Property Rights Holder
      尾野亘, 斉藤成達, 出原正康, 木村剛
    • Industrial Property Number
      特願 2014-044463
    • Filing Date
      2014-03-07

URL: 

Published: 2015-06-25  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi