2013 Fiscal Year Final Research Report
Molecular Mechanism of Metabolic Memory via a DNA Methylation and Its Medical Application
| Project/Area Number |
23390240
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
OGAWA Yoshihiro 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (70291424)
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| Co-Investigator(Renkei-kenkyūsha) |
OKANO Masaki 理化学研究所, 発生・再生科学総合研究センター, チームリーダー (50360863)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | DNAメチル化 / 肝臓 / 脂肪酸β酸化 / PPARα / 新規脂肪合成 / 新生仔 |
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| Research Abstract |
In this study, we analyzed epigenetic regulation of lipid metabolism in mouse liver in perinatal periods. In neonatal periods, DNA methylation of de novo lipogenic glycerol-3-phosphate acyltransferase 1 (GPAT1) gene (Gpam) promoter suppresses the gene expression, whereas Gpam expression is induced in the weaning period via a DNA demethylation mechanism. This suggests de novo mouse hepatic lipogenesis in perinatal periods is regulated by DNA methylation. We also analyzed DNA methylation in neonatal mouse liver from the birth to weaning. It revealed that fatty acid beta-oxidation enzymes were highlighted among the genes, which showed DNA demethylation and increased gene expression in postnatal periods. We also demonstrated that the DNA demethylation is peroxisome proliferator-activated receptor (PPAR)-alpha dependent. This study shed a light on the importance of intrinsic lipid ligands in milk in the epigenetic regulation of lipid metabolism in perinatal mouse liver.
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[Journal Article] Association between body weight at weaning and remodeling in the subcutaneous adipose tissue of obese adult mice with undernourishment in utero2013
Author(s)
Kohmura YK, Kanayama N, Muramatsu K, Tamura N, Yaguchi C, Uchida T, Suzuki K, Sugihara K, Aoe S, Sasaki T, Suganami T, Ogawa Y, Itoh H
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Journal Title
Reprod. Sci
Volume: 20
Pages: 813-827
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[Journal Article] Role of DNA methylation in the regulation of lipogenic glycerol-3-phosphate acyltransferase 1 gene expression in the mouse neonatal liver2012
Author(s)
Ehara T, Kamei Y, Takahashi M, Yuan X, Kanai S, Tamura E, Tanaka M, Yamazaki T, Miura S, Ezaki O, Suganami T, Okano M, Ogawa Y
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Journal Title
Diabetes
Volume: 61
Pages: 2442-2450
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