Diversity and evolution of a canonical mitochondrion-lacking protist group, Fornicata

2013 Fiscal Year Final Research Report

• Project/Area Number: 23405013
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Biodiversity/Systematics
• Research Institution: University of Tsukuba
• Principal Investigator: HASHIMOTO Tetsuo 筑波大学, 生命環境系, 教授 (50208451)
• Co-Investigator(Kenkyū-buntansha): ISHIDA Kenichiro 筑波大学, 生命環境系, 教授 (30282198) ; INAGAKI Inagaki 筑波大学, 生命環境系, 准教授 (50387958)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: フォルニカータ / 嫌気・微好気環境 / 退化型ミトコンドリア / 新奇真核微生物 / 培養株

Research Abstract

Fornicates are a micro aerophilic or anaerobic flagellate group of which diversity has been revealed by environmental DNA analyses. All fornicate organisms whose cellular structures were investigated up to recently have no canonical mitochondria with aerobic respiration, but instead have reduced mitochondria, suggesting that fornicates are an appropriate model system to understand cellular evolution of the reducing process of mitochondria. However, since there still are a few fornicate organisms which have already been isolated and cultured in laboratories, we tried to establish novel cultures for fornicate organisms by exploring natural environments. In this project, we established more than 10 novel cultures for micro aerophilic or anaerobic eukaryotic organisms including several fornicates, and we described a part of them.

Research Products

• [Journal Article] Palpitomonas bilix represents a basal cryptist lineage : Insight into the character evolution in Cryptista (2014)
  • Author(s): Yabuki A, Kamikawa R, Ishikawa SA, Kolisko M, Kim E, Tanabe AS, Kume K, Ishida K, Inagaki Y
  • Journal Title: Scientific Reports Volume: 4 Pages: 4641
  • DOI: 10.1038/srep04641
  • Peer Reviewed
• [Journal Article] Gene-content evolution in discobid mitochondria deduced from the phylogenetic position and complete mitochondrial genome of Tsukubamonas globosa (2014)
  • Author(s): Kamikawa R, Kolisko M, Nishimura Y, Yabuki A, Brown MW, Ishikawa SA, Ishida K, Roger AJ, Hashimoto T, Inagaki Y
  • Journal Title: Genome Biology and Evolution Volume: 6 Pages: 306-315
  • Peer Reviewed
• [Journal Article] Parallel re-modeling of EF-1α function in eukaryotic evolution : Divergent, low-expressed EF-1α genes co-occur with EFL genes in diverse distantly related eukaryotes (2013)
  • Author(s): Kamikawa R, Brown MW, Nishimura Y, Sako Y, Heiss AA, Yubuki N, Gawryluk R, Simpson AGB, Roger AJ, Hahimoto T, Inagaki Y
  • Journal Title: BMC Evol Biol Volume: 13 Pages: 131
  • DOI: 10.1186/1471-2148-13-131
  • Peer Reviewed
• [Journal Article] Multigene phylogenies of diverse Carpediemonas-like organisms identify the closest relatives of 'amitochndriate' diplomonads and retortamonads (2012)
  • Author(s): Takishita K, Kolisko M, Komatsuzaki H, Yabuki A, Inagaki Y, Cepicka I, Smejkalova P, Silberman JD, Hashimoto T, Roger AJ, Simpson AGB
  • Journal Title: Protist Volume: 163 Pages: 344-355
  • Peer Reviewed
• [Journal Article] Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina (2012)
  • Author(s): Nishimura Y, Kamikawa R, Hashimoto T, Inagaki Y
  • Journal Title: PLoS One Volume: 7(5) Pages: e37307
  • DOI: 10.1371/journal.pone.0037307
  • Peer Reviewed
• [Journal Article] RY-coding and non-homogeneous models can ameliorate the maximum-likelihood inferences from nucleotide sequence data with parallel compositional heterogeneity (2012)
  • Author(s): Ishikawa SA, Inagaki Y, Hashimoto T
  • Journal Title: Evolutionary Bioinformatics Volume: 8 Pages: 357-371
  • Peer Reviewed
• [Presentation] 生命の樹-昔と今 (2013)
  • Author(s): 橋本哲男
  • Organizer: 日本進化学会第15回大会進化生物学夏の学校
  • Place of Presentation: つくば
  • Year and Date: 2013-08-31
• [Presentation] A novel endomyxan amoeboflagellate isolated from Pasific ocean (2013)
  • Author(s): Shiratori T, Ishida K
  • Organizer: International Congress of Protistology (ICOP) XIV
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2013-07-28
• [Presentation] 嫌気性寄生原虫におけるエネルギー代謝関連酵素の分子進化 (2013)
  • Author(s): 橋本哲男, 神川龍馬, 林奈々子, 稲垣祐司
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-29
• [Presentation] 嫌気環境に適応したフォルニカータ生物群におけるミトコンドリア関連オルガネラのトランスクリプトーム比較解析 (2012)
  • Author(s): 神 川龍馬, Kolisko M, Andersson J, Simpson AGB, Roger AJ, 稲垣祐司, 橋本哲男
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] フォルニカータ生物群におけるミトコンドリア関連オルガネラの縮退進化-ハイドロジェノソームからマイトソームへ. (2012)
  • Author(s): 神 川龍馬, Kolisko M, Andersson J, Simpson AGB, Roger AJ, 稲垣祐司, 橋本哲男
  • Organizer: 第72回日本寄生虫学会東日本支部会・第10回分子寄生虫マラリアフォーラム合同大会
  • Place of Presentation: 前橋
  • Year and Date: 2012-10-12
• [Presentation] A new strategy of organellar genome sequencing incorporating rolling circle amplification : protist mitochondria genomes (2012)
  • Author(s): Nishimura Y, Kamikawa R, Inagaki Y, Hashimoto T
  • Organizer: Protist 2012
  • Place of Presentation: Oslo
  • Year and Date: 2012-07-29