2013 Fiscal Year Final Research Report
A study of the correlation between hippocampal sclerosis of temporal lobe epilepsy and focal cortical dysplasia
| Project/Area Number |
23500498
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kyushu University |
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Principal Investigator |
TAKASE Kei-ichiro 九州大学, 医学(系)研究科(研究院), 研究員 (00467903)
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| Co-Investigator(Kenkyū-buntansha) |
SHIGETO Hiroshi 九州大学, 大学病院, 講師 (50335965)
KAMADA Takashi 九州大学, 大学病院, 術研究員 (70614460)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 実験てんかん / 皮質異形性 / 側頭葉てんかん / 海馬硬化症 |
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| Research Abstract |
On embryonic day (E) 18, a frozen probe was placed on the frontal scalp of a Sprague-Dawley rat embryo through the uterus wall to produce multiple (two to four) freeze lesions on each hemisphere. Rats were divided into group A (four freeze lesions) and group B (two freeze lesions). EEG video monitoring for rat pups was performed from postnatal day (P) 35 to P77. Brain tissue was examined by immunohistochemistry, including semi-quantitative densitometry. 68.8% of the rats with multiple FCDs showed spontaneous hippocampal seizures which began by movement cessation and culminated in wet-dog shakes during periods of hippocampal discharge, from P47 onwards. Furthermore, immunoreactivities for N-methyl-D-aspartate receptors and glutamate transporters both in FCD lesions and in hippocampi were significantly enhanced compared to controls. Prenatal freeze-lesioning of the brain during embryonic development leads to spontaneous temporal lobe seizures in a rat model with multiple FCDs.
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Research Products
(19 results)
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[Journal Article] fosB-null mice display impaired adult hippocampal neurogenesis and spontaneous epilepsy with depressive behavior2013
Author(s)
Yutsudo N, Kamada T, Kajitani K, Nomaru H, Katogi A, Ohnishi YH, Ohnishi YN, Takase K, Sakumi K, Shigeto H, Nakabeppu Y
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Journal Title
Neuropsychopharmacology
Volume: 38
Pages: 895-906
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[Journal Article] Peri-orbital electrodes as a supplemental recording for detection of ictal discharges in medial temporal lobe epilepsy2011
Author(s)
Shigeto H, Sakata A, Morioka T, Takase K, Hagiwara K, Kamada T, Kanamori Y, Hashiguchi K, Tobimatsu S, Yamashita N, Kira J
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Journal Title
Neurology Asia
Volume: 16
Pages: 303-307
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