Development of a method for simultaneous detection of 4 opportunistic pathogen in mouse

2013 Fiscal Year Final Research Report

• Project/Area Number: 23500500
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Iwate Medical University
• Principal Investigator: KEN-ICHI Hanaki 岩手医科大学, 医学部, 准教授 (40376421)
• Project Period (FY): 2011 – 2013
• Keywords: 診断 / 検査 / マウス / 日和見感染症 / 等温遺伝子増幅法

Research Abstract

Opportunistic organisms, which rarely cause infections in immunocompetent mice, can cause infections in immunocompromised mice. Since opportunistic infection may alter research results using immunocompromised mice, health certificate showing the immunocompromised mouse colony is free from specific opportunistic pathogens is important for biomedical researchers.
The purpose of this study is development of simple and rapid diagnostic loop-mediated isothermal amplification (LAMP) methods for detecting 4 opportunistic pathogens: Staphylococcus aureus (Sa), mouse hepatitis virus (MHV), murine norovirus (MNV), and Pasteurella pneumotropica (Pp). Reverse transcription-LAMP methods for the detection of MHV and MNV have been developed and published in the Journal of Virological Methods. LAMP methods for the detection of antiseptic- and methicillin-resistant Sa had also been developed. However, LAMP method for the detection of Pp has been under construction.

Research Products

• [Journal Article] Detection of murine norovirus by reverse transcription loop-mediated isothermal amplification (2014)
  • Author(s): Hanaki, K., Ike, F., Kajita, A., Yasuno, W., Yanagiba, M., Goto, M., Sakai, K., Ami, Y., Kyuwa, S
  • Journal Title: J Virol Methods Volume: 204C Pages: 17-24
  • DOI: 10.1016/j.jviromet.2014.03.025
• [Journal Article] A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus (2014)
  • Author(s): Hanaki, K., Ike, F., Kajita, A., Yasuno, W., Yanagiba, M., Goto, M., Sakai, K., Ami, Y., Kyuwa, S
  • Journal Title: PLoS One Volume: 9 Pages: e98108
  • DOI: 10.1371/journal.pone.0098108
• [Journal Article] Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses (2013)
  • Author(s): Hanaki, K., Ike, F., Hatakeyama, R., Hirano, N
  • Journal Title: J Virol Methods Volume: 187 Pages: 222-227
  • DOI: 10.1016/j.jviromet.2012.10.008
• [Presentation] マウスノロウイルスを検出するリアルタイム RT-PCR 法の検討 (2013)
  • Author(s): 花木賢一, 池郁生, 酒井宏治, 網康至, 久和茂
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2013-11-10
• [Presentation] LAMP 法によりマウス糞便から4微生物を一括検出する方法の検討 (2013)
  • Author(s): 花木賢一, 池郁生
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2013-05-15
• [Presentation] RT-LAMP 法によるマウスノロウイルスの検出 (2012)
  • Author(s): 花木賢一, 酒井宏治, 池郁生, 久和茂
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学
  • Year and Date: 2012-09-14
• [Presentation] 平野紀夫RT-LAMP 法によるマウス肝炎ウイルスの検出 (2011)
  • Author(s): 花木賢一, 佐藤綾子, 池郁生
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2011-09-19
• [Presentation] エンベロープを有するRNAウイルスに対する免疫捕捉RT-LAMP 法の検討 (2011)
  • Author(s): 佐藤綾子, 松原和衛, 谷口隆秀, 本多英一, 花木賢一
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2011-09-19
• [Presentation] LAMP 法による黄色ブドウ球菌の薬剤耐性, 消毒薬抵抗性の判別 (2011)
  • Author(s): 花木賢一, 池郁生
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: 東京・タワーホール船堀
  • Year and Date: 2011-05-25
• [Remarks]
  • URL: http://www7b.biglobe.ne.jp/~civs-imu/Center_for_In_vivo_Science/DLAM.html
• [Patent(Industrial Property Rights)] 齧歯類コロナウィルスの検出方法 (2011)
  • Inventor(s): 花木賢一
  • Industrial Property Rights Holder: 学校法人岩手医科大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2011-184596
  • Filing Date: 2011-08-26