Biomechanics of Leukocytes migration on Endothelial Cell Monolayer

2013 Fiscal Year Final Research Report

• Project/Area Number: 23500535
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kawasaki University of Medical Welfare
• Principal Investigator: KATAOKA Noriyuki 川崎医療福祉大学, 医療技術学部, 教授 (20250681)
• Co-Investigator(Kenkyū-buntansha): KAJIYA Fumihiko 川崎医療福祉大学, 医療技術学部, 教授 (70029114)
• Project Period (FY): 2011 – 2013
• Keywords: 血管内皮細胞 / 単球 / アクチンフィラメント / アテローム性動脈硬化

Research Abstract

It is well known that one of the critical events in early atherosclerosis is the recruitment of blood monocytes to proatherogenic vascular regions and subsequent transmigration across vascular endothelial cells. To elucidate this phenomenon, we have observed the effects of cytoskeletal structure. Human umbilical vein endothelial cells (Huvecs) were cultured on the porous membrane and/or cell culture dishes. Huvecs were stimulated with IL-1beta and oxLDL. Then monocytes isolated from human blood were added to Huvec monolayer, counted migrated monocytes underneath Huvecs and observed monocytes behavior. When Huvecs were treated with cytochalasin D which blocks polymerization and the elongation of actin, migrated monocytes were decreased. That means that actin filaments are important cytosolic structure for monocytes trans-endothelial migration. In addition, Ellagic Acid hydrolyzed from Ellagitannin which is bioactive polyphenols from pomegranate, has been shown anti-atherogenic effect.

Research Products

• [Journal Article] Increased Passive Stiffness of Cardiomyocytes in the Transverse Direction and Residual Actin and Myosin Cross-Bridge Formation in Hypertrophied Rat Hearts Induced by Chronic beta-Adrenergic Stimulation (2013)
  • Author(s): Sumita Yoshikawa W, Nakamura K, Miura D, Shimizu J, Hashimoto K, Kataoka N, Toyota H, Okuyama H, Miyoshi T, Morita H, Fukushima Kusano K, Matsuo T, Takaki M, Kajiya F, Yagi N, Ohe T, Ito H
  • Journal Title: Circulation Journal Volume: 77(3) Pages: 741-748
  • Peer Reviewed
• [Journal Article] Live-cell visualization of the trans-cellular mode of monocyte transmigration across the vascular, endothelium, and its relationship with endothelial PECAM-1 (2012)
  • Author(s): K. Hashimoto, N. Kataoka, E. Nakamura, K. Hagihara, T. Okamoto, H. Kanouchi, S. Mohri, K. Tsujioka, F. Kajiya
  • Journal Title: The Journal of Physiological Sciences Volume: 62(1) Pages: 63-69
  • Peer Reviewed
• [Presentation] アクチン結合ペプチドLifeact を用いた遊走白血球中のアクチンダイナミクス観察 (2013)
  • Author(s): 片岡則之, 橋本謙, 坂元尚哉, 岡本威明, 毛利聡
  • Organizer: 日本機械学会第25回バイオエンジニアリング講演会
  • Place of Presentation: 産業技術総合研究所 つくばセンター(茨城県つくば市)
  • Year and Date: 2013-10-11
• [Presentation] 動脈硬化発生における細胞・分子のナノ/マイクロメカニクス (2013)
  • Author(s): 片岡則之, 橋本謙
  • Organizer: 日本機械学会年次大会
  • Place of Presentation: 岡山大学(岡山県岡山市)
  • Year and Date: 2013-09-10
• [Presentation] Realtime observation of actindynamics in living and migrating monocytic cells with Lifeact-GFP fusion protein (2012)
  • Author(s): N. Kataoka, K. Hashimoto, T. Okamoto, S. Mohri, F. Kajiya
  • Organizer: Biomedical Engineering Society Annual Meeting
  • Place of Presentation: Atlanta, GA, USA
  • Year and Date: 2012-10-25