Effect of passive smoking on chromosome aberration and DNA damage in alveolar macrophage

2013 Fiscal Year Final Research Report

• Project/Area Number: 23500826
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Kyoto Sangyo University
• Principal Investigator: TAKEUCHI Minoru 京都産業大学, 総合生命科学部, 教授 (70257773)
• Project Period (FY): 2011 – 2013
• Keywords: 受動喫煙 / 副流煙 / 肺胞マクロファージ / 染色体異常 / 遺伝子損傷 / CGHマイクロアレイ

Research Abstract

The influence on health by the passive smoking is an important social problem. In this study, we investigated the effect of passive smoking on the chromosome aberration and DNA damage of alveolar macrophage using passive smoking (cigarette side-stream smoke exposure automatic machine) facility. After smoking, alveolar macrophage was recovered from mice. Chromosome aberration and DNA damage of alveolar macrophages were examined by CGH assay. The significant enhancements of chromosome aberrations were 116 parts, and the deficiencies of chromosome aberrations were 169 parts. Based on these data, we extracted the genome of 10 parts of significantly aberrations. The significantly amplified genome parts were qE1 of chr8, qF2 of chrX, qG1 of chr6, qA3 of chr17. On the contrary, the significantly defective genome parts were qE3 of chr3, qC3.1 of chr5, qB3 of chr13 and qB3 of chr16.

Research Products

• [Journal Article] Alveolar macrophage functions and DNA damage in cigarette smoke-exposed mice (2013)
  • Author(s): Hirono Y, Tanahashi Y, Sasaki K, Konno Y, Shirai Y, Kobayashi K, Someya A, Inaga S, Sakura M, Pinkerton KE, Takeuchi M
  • Journal Title: ABB Volume: 4 Pages: 1-7
  • DOI: 10.4236/abb.2013.48A3001
  • Peer Reviewed
• [Journal Article] ジャングルハニーによる抗体産生機能への影響とその機構について (2013)
  • Author(s): 重吉瑛里, 竹内実
  • Journal Title: 京都産業大学論集 自然科学系列 Volume: 第42号 Pages: 21-52
  • URL: http://ci.nii.ac.jp/els/110009660360.pdf?id=ART0010135608&type=pdf&lang=jp&host=cinii&order_no=&ppv_type=0&lang_sw=&no=1398232334&cp=
  • Peer Reviewed
• [Journal Article] Cigarette smoke induce alteration of structure and function in alveolar macrophages (2013)
  • Author(s): Hirono Y, Kawazoe A, Nose M, Sakura M, Takeuchi M
  • Journal Title: IJBBB Volume: 3(2) Pages: 125-8
  • DOI: 10.7763/IJBBB.2013.V3.179
  • Peer Reviewed
• [Journal Article] Spontaneous occurrence of photoageing-like phenotypes in the dorsal skin of old SAMP1 mice, an oxidative stress model (2013)
  • Author(s): Sakura M, Chiba Y, Kamiya E, Furukawa A, Kawamura N, Niwa M, Takeuchi M, Hosokawa M
  • Journal Title: Exp Dermatol Volume: 22(1) Pages: 62-4
  • DOI: 10.1111/exd.12059
  • Peer Reviewed
• [Journal Article] Effect of Hot Water Extract from Agaricus Blazei Murill on Chemotaxis of Neutrophils (2013)
  • Author(s): Miyagawa M, Hirono Y, Kawazoe A, Shigeyoshi E, Nose M, Sakura M, Pinkerton KE, Takeuchi M
  • Journal Title: JCDSA Volume: 3(1A) Pages: 12-7
  • DOI: 10.4236/jcdsa.2013.31A003
  • Peer Reviewed
• [Journal Article] Environmental mutagens may be implicated in the emergence of drug-resistant microorganisms (2011)
  • Author(s): Miyahara E, Nishie M, Takumi S, Miyanohara H, Nishi J, Yoshiie K, Oda H, Takeuchi M, Komatsu M, Aoyama K, Horiuchi M, Takeuchi T
  • Journal Title: FEMS Microbiol Lett Volume: 317(2) Pages: 109-16
  • DOI: 10.1111/j.1574-6968.2011.02215
  • Peer Reviewed
• [Journal Article] Jungle Honey Enhances Immune Function and Antitumor Activity (2011)
  • Author(s): Fukuda M, Kobayashi K, Hirono Y, Miyagawa M, Ishida T, Ejiogu EC, Sawai M, Pinkerton KE, Takeuchi M
  • Journal Title: Evid Based Complement Alternat Med Volume: 2011 Pages: 1-7
  • DOI: 10.1093/ecam/nen086
  • Peer Reviewed
• [Presentation] The Mechanism of Lipopolysaccharide (LPS)-Induced Lung Inflammation and Effect of Honey (2014)
  • Author(s): M. Takeuchi, A. Kawazoe, A.Takiguchi, Y. Hirono, K. Sasaki, Y. Tanahashi, M. Sakura
  • Organizer: GulfThoracic Congress 2014
  • Place of Presentation: Dubai
  • Year and Date: 20140313-15
• [Presentation] Effect of Cigarette Smoking on Infiltration of Neutrophils in LPS-induced Lung Inflammation (2013)
  • Author(s): SASAKI Kazuma, HIRONO Yuriko, TANAHASHI Yasuyuki, SAKURA Masaaki, ISHIDA Takahiro, Takeuchi Minoru
  • Organizer: 42th Annual Meeting of Japanese Society for Immunology
  • Place of Presentation: Chiba
  • Year and Date: 20131211-13
• [Presentation] The mechanism of inhibition of apoptosis in alveolar macrophages by cigarette smoke (2013)
  • Author(s): HIRONO Yuriko, SASAKI Kazuma, TANAHASHI Yasuyuki, SAKURA Masaaki, ISHIDA Takahiro, INAGA Sumire, TAKEUCHI Minoru
  • Organizer: 42th Annual Meeting of Japanese Society for Immunology
  • Place of Presentation: Chiba
  • Year and Date: 20131211-13
• [Presentation] Cigarette smoke induces DNA damage and Inhibition of function in alveolar macrophage (2013)
  • Author(s): M Takeuchi, Y Hirono, M Sakura
  • Organizer: 44th Union World Conference on Lung Health
  • Place of Presentation: Paris
  • Year and Date: 20131030-1103
• [Presentation] Pinkerton. Cigarette Smoke as Environmental Factor Induce Inhibition of Immune Functions and DNA damage in Alveolar Macrophages (2013)
  • Author(s): M. Takeuchi, Y. Hirono, M. Nose, S. Inoue, K. Sasaki, KE
  • Organizer: EAACI-WAO Congress
  • Place of Presentation: Milan
  • Year and Date: 20130622-26
• [Presentation] Effect of Cigarette Smoke Exposure on LPS-induced Lung Inflammation in Mice (2013)
  • Author(s): M. Takeuchi, A. Kawazoe, Y. Hirono , M. Nose, S. Inaga, KE. Pinkerton
  • Organizer: ATS 2013 International Conference
  • Place of Presentation: Philadelphia
  • Year and Date: 20130517-22
• [Presentation] Highly Mechanized Nano-Structures With Controlled-Release Targeting Technology Is Safe And Compatible In An Aerosol Model For Enhancing Delivery (2013)
  • Author(s): X. Li, college, M. Xue, H. Aaron, M. Tagmount, M. Takeuchi, E. Eisen, C. Vulpe, J. Zink, Risbud, K.E. Pinkerton
  • Organizer: ATS 2013 International Conference
  • Place of Presentation: Philadelphia
  • Year and Date: 20130517-22
• [Presentation] Cigarette Smoke Induce Alteration of Structure and Function in Alveolar Macrophages (2013)
  • Author(s): Yuriko Hirono, Ayaka Kawazoe, Masahiko Nose, Masaaki Sakura and Minoru Takeuchi
  • Organizer: 2013 3rd International Conference on Bioscience, Biochemistry and Bioinformatics (ICBBB 2013)
  • Place of Presentation: Rome, Italy
  • Year and Date: 20130224-25
• [Presentation] Effects of cyclophosphamide as immunosuppressive drug on Alveolar Macrophages by oral administration in rats (2012)
  • Author(s): Y Hirono, M Nose, A Kawazoe, E Shigeyoshi, K Sasaki, Y Tanahashi, M Sakura, M Takeyoshi, F Saito, Y Akahori, N Imatanaka, M Takeuchi
  • Organizer: The 17th Congress of the APSR
  • Place of Presentation: Hong Kong
  • Year and Date: 20121214-16
• [Presentation] Effect of cigarette smoke on primary immune response to Cryptomeria Japonica pollen in the lung (2012)
  • Author(s): NOSE Masahito, KAWAZOE Ayaka, SHIGEYOSHI Eri, SASAKI kazuma, HIRONO Yuriko, TANAHASHI Yasuyuki, SAKURA Masaaki, TAKEUCHI Minoru
  • Organizer: The 17th Congress of the APSR
  • Place of Presentation: Hong Kong
  • Year and Date: 20121214-16
• [Presentation] Immunotoxic effects by oral gavage of cyclophosphamide or cyclosporine A as immunosuppressive drug in Rats (2012)
  • Author(s): HIRONO Yuriko, NOSE Masahito, KAWAZOE Ayaka, SHIGEYOSHI Eri, SASAKI Kazuma, TANAHASHI Yasuyuki, SAKURA Masaaki, TAKEYOSHI Masahiro, SAITO Fumiyo, AKAHORI Yumi, IMATANAKA Nobuya, TAKEUCHI Minoru
  • Organizer: 41th Annual Meeting of Japanese Society for Immunology
  • Place of Presentation: Kobe
  • Year and Date: 20121205-07
• [Presentation] Effect of cigarette smoke and Cryptomeria Japonica Pollen on immune cells in the lung (2012)
  • Author(s): NOSE Masahito, KAWAZOE Ayaka, SHIGEYOSHI Eri, SASAKI kazuma, HIRONO Yuriko, TANAHASHI Yasuyuki, SAKURA Masaaki, TAKEUCHI Minoru
  • Organizer: 41th Annual Meeting of Japanese Society for Immunology
  • Place of Presentation: Kobe
  • Year and Date: 20121205-07
• [Presentation] Effect of cigarette smoking on infiltration of neutrophils to pulmonary by LPS (2012)
  • Author(s): SASAKI Kazuma, KAWAZOE Ayaka, HIRONO Yuriko, NOSE Masahito, SHIGEYOSHI Eri, TANAHASHI Yasuyuki, SAKURA Masaaki, TAKEUCHI Minoru
  • Organizer: 41th Annual Meeting of Japanese Society for Immunology
  • Place of Presentation: Kobe
  • Year and Date: 20121205-07
• [Presentation] CHEMOTACTIC ACTIVITY FOR NEUTROPHILS BY HOT WATER EXTRACT FROM AGARICUS BLAZEI MURILL (2012)
  • Author(s): M. Takeuchi, M. Miyagawa, Y. Hirono, M. Sakura, Y. Tanahashi, H.Takakuwa, K. Outsuki, K.E. Pinkerton
  • Organizer: International Symposium The Neutrophil in Immunity 2012
  • Place of Presentation: Québec
  • Year and Date: 20120609-12
• [Presentation] Delivery Of Nano-Drug Vectors To The Lung : Aerosolization, Fate And Pulmonary Toxicity Of Mesoporous Silica Nanocages (2012)
  • Author(s): X. Li, M. Xue, J. Evans, F. Hayes, H. Aaron, M. Takeuchi, S. Inaga, J. Zink, S. Risbud, K.E. Pinkerton
  • Organizer: ATS 2012 International Conference
  • Place of Presentation: San Francisco
  • Year and Date: 20120518-23
• [Presentation] Effect Of Side-Stream Tobacco Smoke (STS) On Immune Functions And DNA Damage In Alveolar Macrophage (AM) (2012)
  • Author(s): M. Takeuchi, S. Inoue, Y. Hirono, A. Kawazoe, E. Shigeyoshi, M. Nose, S. Inaga, K.E. Pinkerton
  • Organizer: ATS 2012 International Conference
  • Place of Presentation: San Francisco
  • Year and Date: 20120518-23
• [Presentation] Inhibition of Immune Functions and Induction of DNA Damage in Alveolar Macrophage (AM) by Side-stream Tobacco Smoke (2012)
  • Author(s): M. Takeuchi, S. Inoue, Y. Hirono, A. Kawazoe, E. Shigeyoshi, M. Nose
  • Organizer: 15th WCTOH (WORLD CONFERENCE ON TOBACCO OR HEALTH)
  • Place of Presentation: Singapore
  • Year and Date: 20120320-24
• [Presentation] Effect of Cigarette Smoke and Avian Influenza Virus on Alveolar Macrophages (2011)
  • Author(s): HIRONO Yuriko, KAWAZOE Ayaka, SHIGEYOSHI Eri, NOSE Masahito, SAKURA Masaaki, INAGA Sumire, TAKAKUWA Hiroki, OTSUKI Koichi, TAKEUCHI Minoru
  • Organizer: 40thAnnual Meeting of Japanese Society for Immunology
  • Place of Presentation: Makuhari
  • Year and Date: 20111127-29
• [Presentation] Effect of Cigarette Smoke on Induced Immune Cells in Lung by Inhalation of Cryptomeria Japonica Pollen (2011)
  • Author(s): Masahito Nose, Ayaka Kawazoe, Eri Shigeyoshi, Yuriko Hirono, Yasuyuki Tanahashi, Masaaki Sakura, Minoru Takeuchi
  • Organizer: 40th Annual Meeting of Japanese Society for Immunology
  • Place of Presentation: Makuhari
  • Year and Date: 20111127-29
• [Presentation] Effect of Jungle Honey on Antibody Production and its Mechanism (2011)
  • Author(s): SHIGEYOSHI Eri, NOSE Masahito, KAWAZOE Ayaka, HIRONO Yuriko, SAKURA Masaaki, TAKEUCHI Minoru
  • Organizer: 40th Annual Meeting of Japanese Society for Immunology
  • Place of Presentation: Makuhari
  • Year and Date: 20111127-29
• [Presentation] Effectof Cigarette Smoking on LPS-Induced Lung Inflammation (2011)
  • Author(s): KAWAZOE Ayaka, SHIGEYOSHI Eri, NOSE Masahito, HIRONO Yuriko, SAKURA Masaaki, TAKEUCHI Minoru
  • Organizer: 40th Annual Meeting of Japanese Society for Immunology
  • Place of Presentation: Makuhari
  • Year and Date: 20111127-29
• [Presentation] Effect of Jungle Honey on Immunological Functions of Alveolar Macrophages and Antibody Production (2011)
  • Author(s): SHIGEYOSHI Eri, NOSE Masahito, KAWAZOE Ayaka, HIRONO Yuriko, SAKURA Masaaki, TAKEUCHI Minoru
  • Organizer: 16th APSR
  • Place of Presentation: Shanghai
  • Year and Date: 20111103-06
• [Presentation] Effect of Cigarette Smoking on LPS-Induced Lung Inflammation (2011)
  • Author(s): KAWAZOE Ayaka, SHIGEYOSHI Eri, NOSE Masahito, HIRONO Yuriko, SAKURA Masaaki, TAKEUCHI Minoru
  • Organizer: 16th APSR
  • Place of Presentation: Shanghai
  • Year and Date: 20111103-06
• [Presentation] The Inhibitory Mechanism Of Apoptosis In DNA Damaged-Alveolar Macrophages By Cigarette Smoke (2011)
  • Author(s): Y. Hirono, A. Kawazoe, E. Shigeyosi, M. Miyagawa, M. Sakura, S. Inaga, O. Mazda, S. Nagai, T. Takeuchi, K.E. Pinkerton, M. Takeuchi
  • Organizer: ATS 2011 International Conference
  • Place of Presentation: Denver
  • Year and Date: 20110513-18
• [Remarks]
  • URL: http://www.cc.kyoto-su.ac.jp/~mtakex/