2013 Fiscal Year Final Research Report
Development of polymer additive that prevents protein aggregation and inactivation
| Project/Area Number |
23550189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | タンパク質 / 凝集 / 高分子 / 溶液添加剤 / 失活 |
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| Research Abstract |
Small additives have been developed that prevents protein aggregation and inactivation. Here, we have developed the polymers that prevent protein aggregation and protein stabilization, and also we have examined those molecular mechanisms. The molecular mechanism of polyethylene glycol as an aggregation suppressor was different mechanism from that of the well-used additive arginine hydrochloride. It was found that cationic polyallylamine and anionic polyacrylic acid bind on protein that stabilizes protein structure in aqueous solution. PEGylated polymer was more favorable as aggregation suppressor for aggregation-prone protein.
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