2013 Fiscal Year Final Research Report
Development of autofocusing system with video-rate camera for electron tomography
| Project/Area Number |
23560024
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Thin film/Surface and interfacial physical properties
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| Research Institution | Osaka University |
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Principal Investigator |
NISHI Ryuji 大阪大学, 超高圧電子顕微鏡センター, 准教授 (40243183)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Kiyokazu 超高圧電子顕微鏡センター, 特任研究員 (50263223)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 電子線トモグラフィー / 超高圧電子顕微鏡 / オートフォーカス / 自動撮影 / 画像鮮鋭度 |
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| Research Abstract |
It is useful to observe the three-dimensional structure of medical and biological samples by using ultrahigh voltage electron microscope tomography. Taking several tens or more images with slightly different tilt angle of sample is necessary, however, a mechanical rotation accuracy is worse than of a few micrometers. Therefore, we accomplished fast taking of the tilt image series with automatic focusing and field position correction. To capture the 61 CCD images with a resolution of 4000 X 4000 pixels is completed in 30 minutes of half of conventional method.
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