2013 Fiscal Year Final Research Report
Functional crosstalk between ARF and Rab small GTPases
| Project/Area Number |
23570162
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
SHIN Hye-Won 京都大学, 薬学研究科(研究院), 准教授 (10345598)
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| Project Period (FY) |
2011 – 2013
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| Keywords | メンブレントラフィック / 小胞輸送 / エンドソーム / 生体膜 / Gタンパク質 |
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| Research Abstract |
Small GTPases of the ARF family, except for ARF6, mainly localize to the Golgi apparatus, where they trigger formation of coated carrier vesicles. I found that the simultaneous depletion of ARF1/ARF3 or ARF1/ARF4 induced extensive tabulation of Rab4- and Rab11-positive recycling endosomes. Moreover, ARF1/ARF3 are redundantly required for recycling pathway from the recycling endosomes to the plasma membrane. On the other hand, ARF1/ARF4 are redundantly required for the retrograde transport of TGN38 and mannose-6-phosphate receptor from recycling endosomes to the the trans-Golgi network (TGN), but was not required for the recycling pathway. It remains important to determine how distinct combinations of ARFs sort cargos targeted to distinct organelles and regulate separate transport processes on the same compartments. ARFs may act within distinct membrane microdomains along with distinct effector proteins.
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[Journal Article] ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus2013
Author(s)
Nakai, W., Kondo, Y., Saitoh, A., Naito, T., Nakayama, K., and Shin, H.-W.
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Journal Title
Mol. Biol. Cell
Volume: 24
Pages: 2570-2581
DOI
Peer Reviewed
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[Journal Article] EFA6 activates Arf6 and participates in its targeting to the Flemming body during cytokinesis2013
Author(s)
Ueda, T., Hanai, A, Takei, T., Kubo, K., Ohgi, M., Sakagami, H., Takahashi, S., Shin, H.-W., and Nakayama, K.
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Journal Title
FEBS Lett.
Volume: 587
Pages: 1617-1623
Peer Reviewed
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[Journal Article] Mitosis-coupled, microtubule-dependent clustering of endosomal vesicles around centrosomes2013
Author(s)
Takatsu, H., Katoh, Y., Ueda, T., Waguri, S., Murayama, T., Takahashi, S., Shin, H.-W., and Nakayama, K.
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Journal Title
Cell Struct. Funct
Volume: 38
Pages: 31-41
DOI
Peer Reviewed
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[Journal Article] ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway to the plasma membrane2012
Author(s)
Kondo, Y., Hanai, A., Nakai, W., Katoh, Y., Nakayama, K., and Shin, H.-W.
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Journal Title
Cell Struct. Funct.
Volume: 37
Pages: 141-154
DOI
Peer Reviewed
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[Journal Article] Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase2012
Author(s)
Nakamura, K., Man, Z., Xie, Y., Hanai, A., Makyio, H., Kawasaki, M., Kato, R., Shin, H.-W., Nakayama, K., and Wakatsuki, S.
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Journal Title
J. Biol. Chem.
Volume: 287
Pages: 25478-25489
DOI
Peer Reviewed
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[Journal Article] Rab11 regulates exocytosis of recycling vesicles at the plasma membrane2012
Author(s)
Takahashi, S., Kubo, K., Waguri, S., Yabashi, A., Shin, H.-W., Katoh, Y., and Nakayama, K.
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Journal Title
J. Cell Sci.
Volume: 125
Pages: 4049-4057
DOI
Peer Reviewed
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[Journal Article] Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis2012
Author(s)
Makyio, H., Ohgi, M., Takei, T., Takahashi, S., Takatsu, H., Katoh, Y., Hanai, A., Ueda, T., Kanaho, Y., Xie, Y., Shin, H.-W., Kamikubo, H., Kataoka, M., Kawasaki, M., Kato, R., Wakatsuki, S., and Nakayama, K.
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Journal Title
EMBO J.
Volume: 31
Pages: 2590-2603
DOI
Peer Reviewed
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[Journal Article] GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis2011
Author(s)
Takashima, K., Saitoh, A., Hirose, S., Nakai, W., Kondo, Y., Takasu, Y., Kakeya, H., Shin, H.-W., and Nakayama, K.
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Journal Title
Cell Struct. Funct.
Volume: 36
Pages: 223-235
DOI
Peer Reviewed
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[Journal Article] ATP9B, a P4-ATPase (a putative aminophospholipid translocase), localizes to the trans-Golgi network in a CDC50-independent manner2011
Author(s)
Takatsu, H., Baba, K., Shima, T., Umino, H., Kato, U., Umeda, M., Nakayama, K., & Shin, H.-W.
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Journal Title
J. Biol. Chem.
Volume: 286
Pages: 38159-38167
DOI
Peer Reviewed
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[Journal Article] Distinct roles of Rab11 and Arf6 in the regulation of Rab11/FIP3/arfophilin-1 localization in mitotic cells2011
Author(s)
Takahashi, S., Takei, T., Koga, H., Takatsu, H., Shin, H.-W., and Nakayama, K.
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Journal Title
Genes Cells
Volume: 16
Pages: 938-950
DOI
Peer Reviewed
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