2013 Fiscal Year Final Research Report
The functional analysis of vacuolar-type proton pump ATPase
| Project/Area Number |
23570171
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKANISHI Mayumi (20270506)
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| Co-Investigator(Renkei-kenkyūsha) |
FUTAI Asamitsu (50012646)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 酵素の触媒機構 |
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| Research Abstract |
The proton (H+) pumping vacuolar-type ATPase (V-ATPase) is a rotary enzyme comprising multiple subunits that plays a pivotal role in forming intracellular acidic compartments in eukaryotic cells. We have recorded the rotation speed of the V-ATPase using a single molecule analysis system for the first time. We have also identified a temperature-sensitive amino acid motif that is conserved in mammalian V-ATPase E subunits.
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