2013 Fiscal Year Final Research Report
Narrowing the genomic regions responsible for sex determination in spinach
| Project/Area Number |
23580001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Hokkaido University |
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Principal Investigator |
ONODERA Yasuyuki 北海道大学, (連合)農学研究科(研究院), 講師 (80374619)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 植物育種 / F1ハイブリッド |
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| Research Abstract |
Spinach is widely known to be dioecious. However, monoecious plants can also occur in this species. Sex expression in dioecious spinach plants is controlled by a single gene pair termed X and Y. Our previous study showed that a single, incompletely dominant gene, which controls the monoecious condition, is linked to X/Y.
The four Y-linked AFLP markers developed in this study were converted into sequence-characterized amplified region (SCAR) markers. Linkage analysis using the four SCAR markers and three already-known Y-linked markers suggested that recombination is suppressed around the male-determining gene (Y). Analysis of spinach genomic BAC clones isolated with the Y-linked markers suggested that the male-specific (non-recombining) region is at least larger than 840 kbp. Further, most part of this chromosomal region was found to be accounted for by interspersed repeated elements, e.g. retroposons, while only a few protein-coding genes were identified in this region.
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