Analysis on allergenic 13S globulin subunits of buckwheat

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580020
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Crop science/Weed science
• Research Institution: Kyoto University
• Principal Investigator: TANAKA Tomoyuki (勝部 朋之) 京都大学, (連合)農学研究科(研究院), 准教授 (50224473)
• Project Period (FY): 2011 – 2013
• Keywords: ソバ / 13Sグロブリン / 反復配列

Research Abstract

Buckwheat genomic DNA library, consisting of 142,005 clones, was screened to elucidate the diversity of 13S globulin genes. Twenty-six BAC clones were isolated and 17 genes of 13S globulin were identified, the sequences of which were deposited into DNA Data Bank of Japan. The unit length of nucleotides for most tandem repeat sequences, which are specifically inserted into alpha polypeptides of 13S globulin, was 45 bp while that of some repeats had extra three nucleotides like CGG or AGA, resulting in different secondary structure formation. On the other hand, preliminary experimental system was developed to analyze the effect of environmental factors on 13S globulin composition using autogamous tartary buckwheat.

Research Products

• [Journal Article] "反復配列を持たない13Sグロブリン"を低減化したソバ個体の育成-0回反復サブユニットの遺伝子識別技術の開発- (2014)
  • Author(s): 田中朋之・中川真梨子・佐野まどか・安井康夫
  • Journal Title: 作物研究 Volume: 59(印刷中)
  • URL: http://ci.nii.ac.jp/vol_issue/nels/AA12344964_ja.html
  • Peer Reviewed
• [Journal Article] Diversification of 13S globulins, allergenic seed storage proteins, of common buckwheat (2014)
  • Author(s): Sano, M., Nakagawa, M., Oishi, A., Yasui, Y., Katsube-Tanaka, T
  • Journal Title: Food Chemistry Volume: 155 Pages: 192–198
  • DOI: 10.1016/j.foodchem.2014.01.047
  • Peer Reviewed
• [Journal Article] Tandem repeat inserts in 13S globulin subunits, the major allergenic storage protein of common (2012)
  • Author(s): Khan, N., Takahashi, Y., Katsube-Tanaka, T
  • Journal Title: Food Chemistry Volume: 133 Pages: 29-37
  • DOI: 10.1016/j.foodchem.2011.12.056
  • Peer Reviewed
• [Presentation] Allergenic protein, 13S globulin, of common buckwheat diversified by the insertion of tandem repeat (2013)
  • Author(s): T. Katsube-Tanaka
  • Organizer: The 2013 meeting of Korean Society of Crop Science
  • Place of Presentation: Pyeongchang, Korea
  • Year and Date: 20131016-19
• [Presentation] ソバ13Sグロブリン遺伝子の多様性評価 (2013)
  • Author(s): 佐野まどか・中川真梨子・安井康夫・田中朋之
  • Organizer: 日本作物学会
  • Place of Presentation: 鹿児島大学,鹿児島
  • Year and Date: 20130910-12
• [Presentation] Diversity of 13S globulin genes identified in a genomic DNA library of common buckwheat (2013)
  • Author(s): T. Katsube-Tanaka, M. Sano, M. Nakagawa, Y. Yasui
  • Organizer: The 12th International Symposium on Buckwheat
  • Place of Presentation: Laško, Slovenia
  • Year and Date: 20130821-26
• [Presentation] "反復配列を持たない13Sグロブリン遺伝子"を欠失したソバ個体の探索 (2013)
  • Author(s): 田中朋之・中川真梨子・佐野まどか・安井康夫
  • Organizer: 近畿作物・育種研究会第175回例会
  • Place of Presentation: 近畿大学,和歌山
  • Year and Date: 2013-07-13
• [Presentation] ゲノムライブラリーを用いたソバ13Sグロブリンにおける反復挿入配列の解析 (2012)
  • Author(s): 佐野まどか・中川真梨子・安井康夫・田中朋之
  • Organizer: 日本作物学会
  • Place of Presentation: 東北大学,仙台
  • Year and Date: 20120910-11
• [Presentation] Analysis of the major seed storage protein, 13S globulin, in common buckwheat (Fagopyrum esculentum Moench) (2011)
  • Author(s): T. Katsube-Tanaka, N. Khan, Y. Takahashi, M. Nakagawa
  • Organizer: The 7th Asian Crop Science Conference
  • Place of Presentation: Bogor, Indonesia
  • Year and Date: 20110927-30
• [Book] Methods in Molecular Boilogy, vol. 984, N. Volpi and F. Maccari (Eds.), Capillary Electrophoresis of Biomolecules, Capillary electrophoresis of seed strage proteins : the separation and identification of microheterogeneous rice glutelin subunits (2013)
  • Author(s): Katsube-Tanaka T
  • Total Pages: pp. 253-261, (pp. 1-358)
  • Publisher: Humana Press (Springer)
• [Remarks]
  • URL: http://www.kais.kyoto-u.ac.jp/japanese/