2013 Fiscal Year Final Research Report
The oxidation of acetaldehyde: an issue remained unclear in the acetic acid fermentation
| Project/Area Number |
23580115
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
YAKUSHI Toshiharu 山口大学, 医学(系)研究科(研究院), 准教授 (30324388)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUSHITA Kazunobu 山口大学, 農学部, 教授(特命) (50107736)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 酢酸発酵 / 酢酸菌 / アセトアルデヒド / アルデヒド脱水素酵素 / パラログ / 膜タンパク質 / ユビキノン / 遺伝子破壊 |
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| Research Abstract |
Acetic acid fermentation by acetic acid bacteria is a typical microbial transformation from ethanol to acetic acid. The present study conducted genetic and biochemical analyses on aldehyde dehydrogenase (ALDH) catalyzing oxidation of acetaldehyde into acetic acid in Acetobacter pasteurianus, a member of acetic acid bacteria. It was shown that A. pasteurianus has two kinds of ALDH complexes: canonical one is essential for acetic acid fermentation, but paralogous one less contributes to that. Two ALDH complexes were different from each other as to pH optima and affinities to acetaldehyde. The paralogous ALDH was purified as complex consists of three subunits, but canonical ALDH seemed to be unstable and prone to be decomposed into subunit level.
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